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Literature summary for 3.8.1.5 extracted from

  • Bosma, T.; Pikkemaat, M.G.; Kingma, J.; Dijk, J.; Janssen, D.B.
    Steady-state and pre-steady-state kinetic analysis of halopropane conversion by a Rhodococcus haloalkane dehalogenase (2003), Biochemistry, 42, 8047-8053.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus rhodochrous

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state and pre-steady-state kintic analysis, kinetic mechanism of dehalogenation Rhodococcus rhodochrous
0.005
-
1,3-dibromopropane pH 9.4, 30°C Rhodococcus rhodochrous
0.3
-
1,2,3-tribromopropane pH 9.4, 30°C Rhodococcus rhodochrous
0.6
-
1-Bromopropane pH 9.4, 30°C Rhodococcus rhodochrous
0.63
-
1,3-Dichloropropane pH 9.4, 30°C Rhodococcus rhodochrous
1
-
1,2-dibromopropane pH 9.4, 30°C Rhodococcus rhodochrous
1
-
1-Chloropropane pH 9.4, 30°C Rhodococcus rhodochrous
2.2
-
1,2,3-trichloropropane pH 9.4, 30°C Rhodococcus rhodochrous
3.3
-
3-bromo-1-propanol pH 9.4, 30°C Rhodococcus rhodochrous
4
-
1,2-Dibromoethane pH 9.4, 30°C Rhodococcus rhodochrous
11
-
2-bromoethanol above, pH 9.4, 30°C Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
enzyme DhaA
-
Rhodococcus rhodochrous NCIMB 13064
-
enzyme DhaA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Rhodococcus rhodochrous

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,3-tribromopropane + H2O
-
Rhodococcus rhodochrous 2,3-dibromo-1-propanol + bromide
-
?
1,2,3-tribromopropane + H2O
-
Rhodococcus rhodochrous NCIMB 13064 2,3-dibromo-1-propanol + bromide
-
?
1,2,3-trichloropropane + H2O low activity Rhodococcus rhodochrous 2,3-dichloro-1-propanol + chloride
-
?
1,2,3-trichloropropane + H2O low activity Rhodococcus rhodochrous NCIMB 13064 2,3-dichloro-1-propanol + chloride
-
?
1,2-dibromoethane + H2O
-
Rhodococcus rhodochrous 2-bromo-1-ethanol + bromide
-
?
1,2-dibromopropane + H2O
-
Rhodococcus rhodochrous 2-bromo-1-propanol + bromide
-
?
1,2-dichloropropane + H2O
-
Rhodococcus rhodochrous 2-chloro-1-propanol + chloride
-
?
1,3-dibromopropane + H2O best substrate, the release of product 3-bromo-1-propanol is the rate limiting step Rhodococcus rhodochrous 3-bromo-1-propanol + bromide
-
?
1,3-dichloropropane + H2O
-
Rhodococcus rhodochrous 3-chloro-1-propanol + chloride
-
?
1-bromopropane + H2O
-
Rhodococcus rhodochrous propanol + bromide
-
?
1-bromopropane + H2O
-
Rhodococcus rhodochrous NCIMB 13064 propanol + bromide
-
?
1-chloropropane + H2O
-
Rhodococcus rhodochrous propanol + chloride
-
?
1-chloropropane + H2O
-
Rhodococcus rhodochrous NCIMB 13064 propanol + chloride
-
?
2-bromoethanol + H2O low activity Rhodococcus rhodochrous ethanol + bromide
-
?
2-bromoethanol + H2O low activity Rhodococcus rhodochrous NCIMB 13064 ethanol + bromide
-
?
3-bromo-1-propanol + H2O
-
Rhodococcus rhodochrous ethanol + bromide
-
?

Synonyms

Synonyms Comment Organism
DhaA
-
Rhodococcus rhodochrous

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rhodococcus rhodochrous

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information rates of reaction steps Rhodococcus rhodochrous
0.08
-
1,2,3-trichloropropane pH 9.4, 30°C Rhodococcus rhodochrous
0.48
-
1-Chloropropane pH 9.4, 30°C Rhodococcus rhodochrous
1.3
-
1,3-Dichloropropane pH 9.4, 30°C Rhodococcus rhodochrous
2.3
-
1,2-dibromopropane pH 9.4, 30°C Rhodococcus rhodochrous
2.7
-
1,3-dibromopropane pH 9.4, 30°C Rhodococcus rhodochrous
2.7
-
1-Bromopropane pH 9.4, 30°C Rhodococcus rhodochrous
3
-
2-bromoethanol above, pH 9.4, 30°C Rhodococcus rhodochrous
3
-
3-bromo-1-propanol pH 9.4, 30°C Rhodococcus rhodochrous
3.6
-
1,2,3-tribromopropane pH 9.4, 30°C Rhodococcus rhodochrous
14.3
-
1,2-Dibromoethane pH 9.4, 30°C Rhodococcus rhodochrous

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
assay at Rhodococcus rhodochrous