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Literature summary for 3.8.1.5 extracted from

  • Kmunicek, J.; Luengo, S.; Gago, F.; Ortiz, A.R.; Wade, R.C.; Damborsky, J.
    Comparative binding energy analysis of the substrate specificity of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 (2001), Biochemistry, 40, 8905-8917.
    View publication on PubMed
Search for more literature for 3.8.1.5

Protein Variants

Protein Variants Comment Organism
F172W steady state kinetics of the mutant compared to the wild-type enzyme Xanthobacter autotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and binding energy analysis, wild-type and mutant F172W Xanthobacter autotrophicus
0.01
-
 1,2-Dibromoethane
-
 Xanthobacter autotrophicus
0.06
-
 1-bromobutane
-
 Xanthobacter autotrophicus
0.3
-
 1-bromohexane
-
 Xanthobacter autotrophicus
0.5
-
 2-bromoacetonitrile
-
 Xanthobacter autotrophicus
0.52
-
 1,2-Dichloroethane
-
 Xanthobacter autotrophicus
1.29
-
 1,2-dibromopropane
-
 Xanthobacter autotrophicus
1.41
-
 1-Chlorohexane
-
 Xanthobacter autotrophicus
2.19
-
 1-Chlorobutane
-
 Xanthobacter autotrophicus
2.19
-
 epibromohydrine
-
 Xanthobacter autotrophicus
2.4
-
 Dibromomethane
-
 Xanthobacter autotrophicus
6.31
-
 2-chloroacetonitrile
-
 Xanthobacter autotrophicus
11
-
 2-bromoethanol
-
 Xanthobacter autotrophicus
12.88
-
 1,2-dichloropropane
-
 Xanthobacter autotrophicus
19.95
-
 2-bromoacetamide
-
 Xanthobacter autotrophicus
47.9
-
 epichlorohydrin
-
 Xanthobacter autotrophicus
100
-
 Dichloromethane
-
 Xanthobacter autotrophicus
100
-
 2-chloroacetamide
-
 Xanthobacter autotrophicus
398
-
 2-Chloroethanol
-
 Xanthobacter autotrophicus

Organism

Organism UniProt Comment Textmining
Xanthobacter autotrophicus P22643 enzyme DhlA
-
Xanthobacter autotrophicus GJ10 P22643 enzyme DhlA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dibromoethane + H2O
-
 Xanthobacter autotrophicus 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O
-
 Xanthobacter autotrophicus GJ10 2-bromoethanol + bromide
-
 ?
1,2-dibromopropane + H2O
-
 Xanthobacter autotrophicus 2-bromopropan-1-ol + bromide
-
 ?
1,2-dibromopropane + H2O
-
 Xanthobacter autotrophicus GJ10 2-bromopropan-1-ol + bromide
-
 ?
1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus 2-chloroethanol + chloride
-
 ?
1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus GJ10 2-chloroethanol + chloride
-
 ?
1,2-dichloropropane + H2O
-
 Xanthobacter autotrophicus 2-chloropropan-1-ol + chloride
-
 ?
1-bromobutane + H2O
-
 Xanthobacter autotrophicus n-butanol + bromide
-
 ?
1-bromohexane + H2O
-
 Xanthobacter autotrophicus n-hexanol + bromide
-
 ?
1-chlorobutane + H2O
-
 Xanthobacter autotrophicus n-butanol + chloride
-
 ?
1-chlorohexane + H2O
-
 Xanthobacter autotrophicus n-hexanol + chloride
-
 ?
2-bromoacetamide + H2O poor substrate Xanthobacter autotrophicus acetamide + bromide
-
 ?
2-bromoacetonitrile + H2O
-
 Xanthobacter autotrophicus acetonitrile + bromide
-
 ?
2-bromoethanol + H2O
-
 Xanthobacter autotrophicus ethanol + bromide
-
 ?
2-bromoethanol + H2O
-
 Xanthobacter autotrophicus GJ10 ethanol + bromide
-
 ?
2-chloroacetamide + H2O poor substrate Xanthobacter autotrophicus acetamide + chloride
-
 ?
2-chloroacetonitrile + H2O
-
 Xanthobacter autotrophicus acetonitrile + chloride
-
 ?
2-chloroethanol + H2O very poor substrate Xanthobacter autotrophicus ethanol + chloride
-
 ?
dibromomethane + H2O
-
 Xanthobacter autotrophicus bromomethanol + bromide
-
 ?
dichloromethane + H2O poor substrate Xanthobacter autotrophicus chloromethanol + chloride
-
 ?
epibromohydrine + H2O
-
 Xanthobacter autotrophicus ?
-
 ?
epichlorohydrin + H2O poor substrate Xanthobacter autotrophicus ?
-
 ?
additional information substrate specificity analysis, overview Xanthobacter autotrophicus ?
-
 ?
additional information substrate specificity analysis, overview Xanthobacter autotrophicus GJ10 ?
-
 ?

Subunits

Subunits Comment Organism
More enzyme-ligand-structure of wild-type and mutant enzymes, overview Xanthobacter autotrophicus

Synonyms

Synonyms Comment Organism
DhlA
-
 Xanthobacter autotrophicus