Cloned (Comment) | Organism |
---|---|
GJ10 mutant H289Q, expression in Escherichia coli | Xanthobacter autotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
H289Q | GJ10, mutant with 660fold reduced activity with substrate 1,2-dibromoethane | Xanthobacter autotrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
halide | GJ10 mutant H289Q blocked by bromide in intermediate state | Xanthobacter autotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
1,2-Dibromoethane | GJ10 | Xanthobacter autotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dichloroethane + H2O | Xanthobacter autotrophicus | - |
2-chloroethanol + chloride | - |
? | |
1,2-dichloroethane + H2O | Xanthobacter autotrophicus GJ10 | - |
2-chloroethanol + chloride | - |
? | |
n-haloalkanes + H2O | Xanthobacter autotrophicus | broad substrate range | primary alcohols + halide | - |
? | |
n-haloalkanes + H2O | Xanthobacter autotrophicus GJ10 | broad substrate range | primary alcohols + halide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthobacter autotrophicus | - |
GJ10 | - |
Xanthobacter autotrophicus GJ10 | - |
GJ10 | - |
Purification (Comment) | Organism |
---|---|
GJ10 mutant H289Q | Xanthobacter autotrophicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-haloalkane + H2O = a primary alcohol + halide | alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction | Xanthobacter autotrophicus | |
1-haloalkane + H2O = a primary alcohol + halide | two-step mechanism involving an ester intermediate covalently bound at Asp124, His 289 is important for hydrolysis | Xanthobacter autotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dibromoethane + H2O | - |
Xanthobacter autotrophicus | 2-bromoethanol + bromide | - |
? | |
1,2-dibromoethane + H2O | - |
Xanthobacter autotrophicus GJ10 | 2-bromoethanol + bromide | - |
? | |
1,2-dichloroethane + H2O | - |
Xanthobacter autotrophicus | 2-chloroethanol + chloride | - |
? | |
1,2-dichloroethane + H2O | - |
Xanthobacter autotrophicus GJ10 | 2-chloroethanol + chloride | - |
? | |
haloalkane + H2O | - |
Xanthobacter autotrophicus | alcohol + halide | - |
? | |
haloalkane + H2O | - |
Xanthobacter autotrophicus GJ10 | alcohol + halide | - |
? | |
n-haloalkanes + H2O | broad substrate range | Xanthobacter autotrophicus | primary alcohols + halide | - |
? | |
n-haloalkanes + H2O | broad substrate range | Xanthobacter autotrophicus GJ10 | primary alcohols + halide | - |
? |