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Literature summary for 3.8.1.5 extracted from

  • Nagata, Y.; Hynkova, K.; Damborsky, J.; Takagi, M.
    Construction and characterization of histidine-tagged haloalkane dehalogenase (LinB) of a new substrate class from a gamma-hexachlorocyclohexane-degrading bacterium, Sphingomonas paucimobilis UT26 (1999), Protein Expr. Purif., 17, 299-304.
    View publication on PubMed
Search for more literature for 3.8.1.5

Application

Application Comment Organism
agriculture biodegradation of 1,2-dichloropropane and 2-chloropropane via modified enzyme Sphingomonas paucimobilis

Cloned(Commentary)

Cloned (Comment) Organism
UT26, His-tagged protein expressed in Escherichia coli Sphingomonas paucimobilis

Protein Variants

Protein Variants Comment Organism
F151W UT26, His-tagged mutant Sphingomonas paucimobilis
W109L UT26, His-tagged mutant, no activity Sphingomonas paucimobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18
-
 1-Chlorobutane UT26, His-tagged purified protein Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O Sphingomonas paucimobilis
-
 2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride
-
 ?
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O Sphingomonas paucimobilis UT26
-
 2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride
-
 ?
n-haloalkanes + H2O Sphingomonas paucimobilis broad substrate range primary alcohols + halide
-
 ?
n-haloalkanes + H2O Sphingomonas paucimobilis UT26 broad substrate range primary alcohols + halide
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis
-
 e.g. Pseudomonas paucimobilis
-
Sphingomonas paucimobilis
-
 UT26
-
Sphingomonas paucimobilis UT26
-
 UT26
-

Purification (Commentary)

Purification (Comment) Organism
UT26, His-tagged protein purified on Ni-NTA resin Sphingomonas paucimobilis

Reaction

Reaction Comment Organism Reaction ID
1-haloalkane + H2O = a primary alcohol + halide alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction Sphingomonas paucimobilis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 comparison of wild-type and His-tagged enzyme concerning reaction rates for several substrates, remaining activity about 80% Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O
-
 Sphingomonas paucimobilis 2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride
-
 ?
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O
-
 Sphingomonas paucimobilis UT26 2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride
-
 ?
1-chlorodecane + H2O
-
 Sphingomonas paucimobilis decanol + chloride
-
 ?
1-chlorodecane + H2O
-
 Sphingomonas paucimobilis UT26 decanol + chloride
-
 ?
2-chlorobutane + H2O
-
 Sphingomonas paucimobilis 2-butanol + chloride
-
 ?
2-chlorobutane + H2O
-
 Sphingomonas paucimobilis UT26 2-butanol + chloride
-
 ?
haloalkane + H2O
-
 Sphingomonas paucimobilis alcohol + halide
-
 ?
haloalkane + H2O
-
 Sphingomonas paucimobilis UT26 alcohol + halide
-
 ?
n-haloalkanes + H2O broad substrate range Sphingomonas paucimobilis primary alcohols + halide
-
 ?
n-haloalkanes + H2O broad substrate range Sphingomonas paucimobilis UT26 primary alcohols + halide
-
 ?