Crystallization (Comment) | Organism |
---|---|
GJ10, X-ray structure of F172W mutant | Xanthobacter autotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
F172W | mutant with 10fold higher KM for 1-chlorohexane and 2fold higher KM for 1,2-dibromoethane, increased bromide release but velocity of hydrolysis of alkyl-enzyme bond remains unchanged | Xanthobacter autotrophicus |
F172Y | mutant with 5fold higher KM for 1-chlorohexane | Xanthobacter autotrophicus |
additional information | GJ10, several mutants of F172, tested for substrate binding | Xanthobacter autotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
1,2-Dichloroethane | GJ10, calculated from rate constants for four-steps reaction mechanism | Xanthobacter autotrophicus | |
0.008 | - |
1,2-Dichloroethane | GJ10, F172W mutant, calculated from rate constants for four-steps reaction mechanism | Xanthobacter autotrophicus | |
4.3 | - |
1,2-Dibromoethane | GJ10, calculated from rate constants for four-steps reaction mechanism | Xanthobacter autotrophicus | |
29 | - |
1,2-Dibromoethane | GJ10, F172W mutant, calculated from rate constants for four-steps reaction mechanism | Xanthobacter autotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dichloroethane + H2O | Xanthobacter autotrophicus | - |
2-chloroethanol + chloride | - |
? | |
1,2-dichloroethane + H2O | Xanthobacter autotrophicus GJ10 | - |
2-chloroethanol + chloride | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthobacter autotrophicus | - |
GJ10 | - |
Xanthobacter autotrophicus GJ10 | - |
GJ10 | - |
Purification (Comment) | Organism |
---|---|
GJ10 | Xanthobacter autotrophicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-haloalkane + H2O = a primary alcohol + halide | GJ10, four-step reaction mechanism with covalent ester intermediate | Xanthobacter autotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dibromoethane + H2O | - |
Xanthobacter autotrophicus | 2-bromoethanol + bromide | - |
? | |
1,2-dibromoethane + H2O | - |
Xanthobacter autotrophicus GJ10 | 2-bromoethanol + bromide | - |
? | |
1,2-dichloroethane + H2O | - |
Xanthobacter autotrophicus | 2-chloroethanol + chloride | - |
? | |
1,2-dichloroethane + H2O | - |
Xanthobacter autotrophicus GJ10 | 2-chloroethanol + chloride | - |
? | |
1-chlorohexane + H2O | - |
Xanthobacter autotrophicus | hexanol + chloride | - |
? | |
1-chlorohexane + H2O | - |
Xanthobacter autotrophicus GJ10 | hexanol + chloride | - |
? | |
haloalkane + H2O | - |
Xanthobacter autotrophicus | alcohol + halide | - |
? | |
haloalkane + H2O | - |
Xanthobacter autotrophicus GJ10 | alcohol + halide | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | GJ10, rate limiting step is hydrolysis of alkyl-enzyme intermediate | Xanthobacter autotrophicus |