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Literature summary for 3.8.1.5 extracted from

  • Yokota, T.; Omori, T.; Kodama, T.
    Purification and properties of haloalkane dehalogenase from Corynebacterium sp. strain m15-3 (1987), J. Bacteriol., 169, 4049-4054.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 1-Chlorobutane
-
 Corynebacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 gel filtration Corynebacterium sp.
36000
-
 1 * 36000, SDS-PAGE Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-
Corynebacterium sp. m15-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strain m15-3 Corynebacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dibromoethane + H2O 42% of the activity with 4-chlorobutanol Corynebacterium sp. 2-bromoethanol + bromide
-
 ?
1,2-dichlorobutane + H2O 7.8% of the activity with 4-chlorobutanol Corynebacterium sp. ?
-
 ?
1,3-dichlorobutane + H2O 12% of the activity with 4-chlorobutanol Corynebacterium sp. ?
-
 ?
1,3-dichloropropane + H2O 21% of the activity with 4-chlorobutanol Corynebacterium sp. ?
-
 ?
1,3-dichloropropane + H2O 21% of the activity with 4-chlorobutanol Corynebacterium sp. m15-3 ?
-
 ?
1,4-dichlorobutane + H2O 27% of the activity with 4-chlorobutanol Corynebacterium sp. ?
-
 ?
1,5-dichloropentane + H2O 20% of the activity with 4-chlorobutanol Corynebacterium sp. ?
-
 ?
1,6-dichlorohexane + H2O 32% of the activity with 4-chlorobutanol Corynebacterium sp. ?
-
 ?
1,7-dichloroheptane + H2O 20% of the activity with 4-chlorobutanol Corynebacterium sp. ?
-
 ?
1,8-dichlorooctane + H2O 32% of the activity with 4-chlorobutanol Corynebacterium sp. ?
-
 ?
1,9-dichlorononane + H2O 14% of the activity with 4-chlorobutanol Corynebacterium sp. ?
-
 ?
1-bromobutane + H2O 25% of the activity with 4-chlorobutanol Corynebacterium sp. butanol + bromide
-
 ?
1-chloro-2-methylpropane + H2O 20% of the activity with 4-chlorobutanol Corynebacterium sp. 2-methylpropanol + chloride
-
 ?
1-chloro-4-methylbutane + H2O 42% of the activity with 4-chlorobutanol Corynebacterium sp. pentanol + chloride
-
 ?
1-chlorobutane + H2O 22% of the activity with 4-chlorobutanol Corynebacterium sp. butanol + chloride
-
 ?
1-chlorobutane + H2O 22% of the activity with 4-chlorobutanol Corynebacterium sp. m15-3 butanol + chloride
-
 ?
1-chloroheptane + H2O 22% of the activity with 4-chlorobutanol Corynebacterium sp. heptanol + chloride
-
 ?
1-chlorohexane + H2O 15% of the activity with 4-chlorobutanol Corynebacterium sp. hexanol + chloride
-
 ?
1-chlorononane + H2O 13% of the activity with 4-chlorobutanol Corynebacterium sp. nonanol + chloride
-
 ?
1-chlorooctane + H2O 18% of the activity with 4-chlorobutanol Corynebacterium sp. octanol + chloride
-
 ?
1-chloropentane + H2O 20% of the activity with 4-chlorobutanol Corynebacterium sp. pentanol + chloride
-
 ?
1-chloropropane + H2O 5% of the activity with 4-chlorobutanol Corynebacterium sp. propanol + chloride
-
 ?
1-chloropropane + H2O 5% of the activity with 4-chlorobutanol Corynebacterium sp. m15-3 propanol + chloride
-
 ?
1-iodobutane + H2O 12% of the activity with 4-chlorobutanol Corynebacterium sp. butanol + iodide
-
 ?
4-chlorobutanol + H2O highest activity Corynebacterium sp. butane-1,4-diol + chloride
-
 ?
bromoethane + H2O 32% of the activity with 4-chlorobutanol Corynebacterium sp. ethanol + bromide
-
 ?
bromoethane + H2O 32% of the activity with 4-chlorobutanol Corynebacterium sp. m15-3 ethanol + bromide
-
 ?
haloalkane + H2O
-
 Corynebacterium sp. alcohol + halide
-
 ?
haloalkane + H2O
-
 Corynebacterium sp. m15-3 alcohol + halide
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Corynebacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
 Corynebacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 30 stable for 1 h Corynebacterium sp.
40
-
 after 1 h 40% loss of activity Corynebacterium sp.
50
-
 after 1 h 88% loss of activity Corynebacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
-
 Corynebacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 8.5 stable for 24 h at 4°C Corynebacterium sp.