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Literature summary for 3.8.1.3 extracted from

  • Nakayama, T.; Kamachi, T.; Jitsumori, K.; Omi, R.; Hirotsu, K.; Esaki, N.; Kurihara, T.; Yoshizawa, K.
    Substrate specificity of fluoroacetate dehalogenase: an insight from crystallographic analysis, fluorescence spectroscopy, and theoretical computations (2012), Chemistry, 18, 8392-8402.
    View publication on PubMed
Search for more literature for 3.8.1.3

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant D104A in compex with fluoroacetate, X-ray diffraction structure determination and analysis at 1.2 A resolution Burkholderia sp.

Protein Variants

Protein Variants Comment Organism
D104A site-directed mutagenesis Burkholderia sp.
W150F site-directed mutagenesis Burkholderia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chloroacetate + H2O Burkholderia sp.
-
 glycolate + chloride
-
 ?
fluoroacetate + H2O Burkholderia sp.
-
 glycolate + fluoride
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia sp. Q1JU72
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chloroacetate + H2O
-
 Burkholderia sp. glycolate + chloride
-
 ?
chloroacetate + H2O substrate binding structure Burkholderia sp. glycolate + chloride
-
 ?
fluoroacetate + H2O
-
 Burkholderia sp. glycolate + fluoride
-
 ?
fluoroacetate + H2O substrate binding structure Burkholderia sp. glycolate + fluoride
-
 ?

Synonyms

Synonyms Comment Organism
fluoroacetate dehalogenase
-
 Burkholderia sp.

General Information

General Information Comment Organism
additional information the enzyme active site is composed of Asp104, Arg105, Arg108, His149, Trp150, Tyr212, and His271, environments of the tryptophan residues are different between the fluoroacetate and chloroacetate-bound enzymes, active site docking simulations and QM/MM optimizations, QM/MM calculations for the defluorination of fluoroacetate, overview Burkholderia sp.