Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | - |
- |
- |
Delftia acidovorans | - |
- |
- |
Delftia acidovorans B | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
haloacetate + H2O = glycolate + halide | reaction mechanism of fluoroacetate dehalogenase, overview. Asp104 serves as nucleophile to attack the alpha-carbon atom of the substrate to displace the fluorine atom leading to the formation of an ester intermediate. The ester intermediate is subsequently hydrolyzed by a water molecule activated by His271, which yields glycosylate and regenerates the carboxylate group of Asp104. The catalytic triad is formed by Asp104-His271-Asp128 | Burkholderia sp. | |
haloacetate + H2O = glycolate + halide | reaction mechanism of fluoroacetate dehalogenase, overview. Asp105 serves as nucleophile to attack the alpha-carbon atom of the substrate to displace the fluorine atom leading to the formation of an ester intermediate. The ester intermediate is subsequently hydrolyzed by a water molecule activated by His272, which yields glycosylate and regenerates the carboxylate group of Asp105 | Delftia acidovorans |
Synonyms | Comment | Organism |
---|---|---|
FAc-Dex FA1 | - |
Burkholderia sp. |
FAc-Dex H1 | - |
Delftia acidovorans |
fluoroacetate dehalogenase | - |
Delftia acidovorans |
fluoroacetate dehalogenase | - |
Burkholderia sp. |
General Information | Comment | Organism |
---|---|---|
additional information | the active site is formed by Phe34, Asp104, Arg105, Arg108, Asp128, His271, and Phe272 of the core domain, as well as of Tyr147, His149, Trp150, and Tyr212 of the cap domain, structure analysis, overview | Burkholderia sp. |