Literature summary for 3.8.1.3 extracted from

Kawasaki, H.; Tone, N.; Tonomura, K.
Purification and properties of haloacetate halidohydrolase specified by plasmid from Moraxella sp. strain B (1981), Agric. Biol. Chem., 45, 35-42.

No PubMed abstract available

Search for more literature for 3.8.1.3

Inhibitors

Inhibitors	Comment	Organism	Structure
AgCl2	-	Moraxella sp.
HgCl2	-	Moraxella sp.
N-ethylmaleimide	-	Moraxella sp.
N-ethylmaleimide	-	Pseudomonas sp.
p-chloromercuribenzoate	-	Moraxella sp.
p-chloromercuriphenylsulfonate	-	Moraxella sp.
p-chloromercuriphenylsulfonate	-	Pseudomonas sp.
thiol reagents	-	Moraxella sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1	-	monoiodoacetate	-	Pseudomonas sp.
1.1	-	monoiodoacetate	-	Moraxella sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	-	gel filtration	Moraxella sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
monobromoacetate + H2O	Pseudomonas sp.	H2	glycolate + HBr	-	ir
monobromoacetate + H2O	Moraxella sp.	H2	glycolate + HBr	-	ir
monochloroacetate + H2O	Pseudomonas sp.	H1,H2	glycolate + HCl	-	ir
monochloroacetate + H2O	Moraxella sp.	H1,H2	glycolate + HCl	-	ir
monofluoroacetate + H2O	Pseudomonas sp.	-	glycolate + HF	-	?
monofluoroacetate + H2O	Moraxella sp.	H1	glycolate + HF	-	ir
monoiodoacetate + H2O	Pseudomonas sp.	H2	glycolate + HI	-	ir
monoiodoacetate + H2O	Moraxella sp.	H2	glycolate + HI	-	ir

Organism

Organism	UniProt	Comment	Textmining
Moraxella sp.	-	-	-
Pseudomonas sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
H2	Moraxella sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
monobromoacetate + H2O	H2	Pseudomonas sp.	glycolate + HBr	-	ir
monobromoacetate + H2O	H2	Moraxella sp.	glycolate + HBr	-	ir
monochloroacetate + H2O	H1,H2	Pseudomonas sp.	glycolate + HCl	-	ir
monochloroacetate + H2O	H1,H2	Moraxella sp.	glycolate + HCl	-	ir
monofluoroacetate + H2O	-	Pseudomonas sp.	glycolate + HF	-	?
monofluoroacetate + H2O	H1	Moraxella sp.	glycolate + HF	-	ir
monoiodoacetate + H2O	H2	Pseudomonas sp.	glycolate + HI	-	ir
monoiodoacetate + H2O	H2	Moraxella sp.	glycolate + HI	-	ir

Subunits

Subunits	Comment	Organism
dimer	-	Pseudomonas sp.
dimer	2 * 26000-27000	Moraxella sp.

Synonyms

Synonyms	Comment	Organism
monohaloacetate dehalogenase	-	-

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Pseudomonas sp.
50	-	-	Moraxella sp.

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	60	-	Pseudomonas sp.
30	60	-	Moraxella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
20	60	-	Pseudomonas sp.
20	60	rapid denaturation over 70°C	Moraxella sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.5	-	-	Pseudomonas sp.
9.5	-	-	Moraxella sp.

pH Range

pH Minimum	pH Maximum	Comment	Organism
8	11	-	Moraxella sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	10	-	Moraxella sp.

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Release 2023.1 (January 2023)