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Literature summary for 3.8.1.2 extracted from

  • Bachas-Daunert, P.G.; Law, S.A.; Wei, Y.
    Characterization of a recombinant thermostable dehalogenase isolated from the hot spring thermophile Sulfolobus tokodaii (2009), Appl. Biochem. Biotechnol., 159, 382-393.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli in a tag-free form Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
L-2-bromohexanoate pH 9.5, 70°C Sulfurisphaera tokodaii
1.7
-
L-2-chloropropionate pH 9.5, 70°C Sulfurisphaera tokodaii
1.7
-
L-2-bromobutanoate pH 9.5, 70°C Sulfurisphaera tokodaii
2.1
-
L-2-bromopropionate pH 9.5, 70°C Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q96XE7
-
-
Sulfurisphaera tokodaii 7 Q96XE7
-
-

Purification (Commentary)

Purification (Comment) Organism
two-step method: heat treatment step that removes 90% of the impurities, followed by an acidification step that eliminates the remaining impurities. The easy purification procedure renders the production of this protein fast, cost effective, and suitable for large-scale production Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-bromobutanoate + H2O the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer Sulfurisphaera tokodaii 2-hydroxybutanoate + HBr
-
?
L-2-bromobutanoate + H2O the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer Sulfurisphaera tokodaii 7 2-hydroxybutanoate + HBr
-
?
L-2-bromohexanoate + H2O the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer Sulfurisphaera tokodaii 2-hydroxyhexanoate + HBr
-
?
L-2-bromohexanoate + H2O the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer Sulfurisphaera tokodaii 7 2-hydroxyhexanoate + HBr
-
?
L-2-bromopropionate + H2O the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer Sulfurisphaera tokodaii lactate + HBr
-
?
L-2-bromopropionate + H2O the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer Sulfurisphaera tokodaii 7 lactate + HBr
-
?
L-2-chloropropionate + H2O the enzyme catalyzes the stereospecific removal of chloride from the L-conformer but not the D-conformer Sulfurisphaera tokodaii lactate + HCl
-
?
L-2-chloropropionate + H2O the enzyme catalyzes the stereospecific removal of chloride from the L-conformer but not the D-conformer Sulfurisphaera tokodaii 7 lactate + HCl
-
?
additional information the enzyme is not active toward 1,4-dibromobutane Sulfurisphaera tokodaii ?
-
?
additional information the enzyme is not active toward 1,4-dibromobutane Sulfurisphaera tokodaii 7 ?
-
?

Synonyms

Synonyms Comment Organism
L-2_HAD
-
Sulfurisphaera tokodaii
STK_25700 locus name Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Sulfurisphaera tokodaii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 90 50°C: about 40% of maximal activity, 90°C: about 80% of maximal activity Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
4 h, enzyme remains fully active Sulfurisphaera tokodaii
80
-
4 h, 10% loss of activity Sulfurisphaera tokodaii
90
-
1 h, 70% loss of activity Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5 10
-
Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
9 11.5 pH 9.0: about 45% of maximal activity, pH 11.5: about 70% of maximal activity Sulfurisphaera tokodaii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 the enzyme tolerates extreme pH conditions ranging from 4 to 10 Sulfurisphaera tokodaii