Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli in a tag-free form | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
L-2-bromohexanoate | pH 9.5, 70°C | Sulfurisphaera tokodaii | |
1.7 | - |
L-2-chloropropionate | pH 9.5, 70°C | Sulfurisphaera tokodaii | |
1.7 | - |
L-2-bromobutanoate | pH 9.5, 70°C | Sulfurisphaera tokodaii | |
2.1 | - |
L-2-bromopropionate | pH 9.5, 70°C | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q96XE7 | - |
- |
Sulfurisphaera tokodaii 7 | Q96XE7 | - |
- |
Purification (Comment) | Organism |
---|---|
two-step method: heat treatment step that removes 90% of the impurities, followed by an acidification step that eliminates the remaining impurities. The easy purification procedure renders the production of this protein fast, cost effective, and suitable for large-scale production | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-bromobutanoate + H2O | the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer | Sulfurisphaera tokodaii | 2-hydroxybutanoate + HBr | - |
? | |
L-2-bromobutanoate + H2O | the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer | Sulfurisphaera tokodaii 7 | 2-hydroxybutanoate + HBr | - |
? | |
L-2-bromohexanoate + H2O | the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer | Sulfurisphaera tokodaii | 2-hydroxyhexanoate + HBr | - |
? | |
L-2-bromohexanoate + H2O | the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer | Sulfurisphaera tokodaii 7 | 2-hydroxyhexanoate + HBr | - |
? | |
L-2-bromopropionate + H2O | the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer | Sulfurisphaera tokodaii | lactate + HBr | - |
? | |
L-2-bromopropionate + H2O | the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer | Sulfurisphaera tokodaii 7 | lactate + HBr | - |
? | |
L-2-chloropropionate + H2O | the enzyme catalyzes the stereospecific removal of chloride from the L-conformer but not the D-conformer | Sulfurisphaera tokodaii | lactate + HCl | - |
? | |
L-2-chloropropionate + H2O | the enzyme catalyzes the stereospecific removal of chloride from the L-conformer but not the D-conformer | Sulfurisphaera tokodaii 7 | lactate + HCl | - |
? | |
additional information | the enzyme is not active toward 1,4-dibromobutane | Sulfurisphaera tokodaii | ? | - |
? | |
additional information | the enzyme is not active toward 1,4-dibromobutane | Sulfurisphaera tokodaii 7 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-2_HAD | - |
Sulfurisphaera tokodaii |
STK_25700 | locus name | Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Sulfurisphaera tokodaii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | 50°C: about 40% of maximal activity, 90°C: about 80% of maximal activity | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
4 h, enzyme remains fully active | Sulfurisphaera tokodaii |
80 | - |
4 h, 10% loss of activity | Sulfurisphaera tokodaii |
90 | - |
1 h, 70% loss of activity | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | 10 | - |
Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 11.5 | pH 9.0: about 45% of maximal activity, pH 11.5: about 70% of maximal activity | Sulfurisphaera tokodaii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | the enzyme tolerates extreme pH conditions ranging from 4 to 10 | Sulfurisphaera tokodaii |