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Literature summary for 3.8.1.2 extracted from

  • Esaki, N.; Kurihara, T.; Li, Y.F.; Ichiyama, S.
    Novel mechanism of enzymatic hydrolysis involving cyanoalanine intermediate revealed by mass spectrometric monitoring of an enzyme reaction (2001), ICR Annual Report, 7, 46-47.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
D10N Asn10 of the mutant enzyme is spontaneously deaminated to yield Asp, though slowly, causing increasing activity of the mutant preparation Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-haloalkanoic acid + H2O Pseudomonas putida
-
D-2-hydroxyalkanoic acid + halide
-
?
L-2-haloalkanoic acid + H2O Pseudomonas putida YL
-
D-2-hydroxyalkanoic acid + halide
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
YL
-
Pseudomonas putida YL
-
YL
-

Reaction

Reaction Comment Organism Reaction ID
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-haloacid + H2O
-
Pseudomonas putida (R)-2-hydroxyacid + halide
-
?
(S)-2-haloacid + H2O
-
Pseudomonas putida YL (R)-2-hydroxyacid + halide
-
?
L-2-haloalkanoic acid + H2O
-
Pseudomonas putida D-2-hydroxyalkanoic acid + halide
-
?
L-2-haloalkanoic acid + H2O
-
Pseudomonas putida YL D-2-hydroxyalkanoic acid + halide
-
?