Protein Variants | Comment | Organism |
---|---|---|
D10N | Asn10 of the mutant enzyme is spontaneously deaminated to yield Asp, though slowly, causing increasing activity of the mutant preparation | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-haloalkanoic acid + H2O | Pseudomonas putida | - |
D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | Pseudomonas putida YL | - |
D-2-hydroxyalkanoic acid + halide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
YL | - |
Pseudomonas putida YL | - |
YL | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide | Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-haloacid + H2O | - |
Pseudomonas putida | (R)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | - |
Pseudomonas putida YL | (R)-2-hydroxyacid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | - |
Pseudomonas putida | D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | - |
Pseudomonas putida YL | D-2-hydroxyalkanoic acid + halide | - |
? |