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Literature summary for 3.8.1.2 extracted from
- Cloning and characterization of a cryptic haloacid dehalogenase from Burkholderia cepacia MBA4 (1999), J. Bacteriol., 181, 6003-6009.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Burkholderia cepacia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | 41% inhibition | Burkholderia cepacia |  |
| HgCl2 | - |
Burkholderia cepacia | |
| p-mercuric chlorobenzoate | - |
Burkholderia cepacia | |
| RbCl2 | - |
Burkholderia cepacia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.13 | - |
monobromoacetate | pH 7.9, 30°C | Burkholderia cepacia | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Burkholderia cepacia | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
2 * 27000, SDS-PAGE | Burkholderia cepacia |
| 58000 | - |
gel filtration | Burkholderia cepacia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-chloropropionate + H2O | Burkholderia cepacia | - |
lactate + HCl | - |
? | |
| L-2-haloalkanoic acid + H2O | Burkholderia cepacia | - |
D-2-hydroxyalkanoic acid + halide | - |
? | |
| monobromoacetate + H2O | Burkholderia cepacia | - |
glycolate + HBr | - |
? | |
| monochloroacetate + H2O | Burkholderia cepacia | - |
glycolate + HCl | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | Q51645 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.56 | - |
- |
Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-2-haloacid + H2O | - |
Burkholderia cepacia | (R)-2-hydroxyacid + halide | - |
? | |
| 2-chloropropionate + H2O | - |
Burkholderia cepacia | lactate + HCl | - |
? | |
| L-2-haloalkanoic acid + H2O | - |
Burkholderia cepacia | D-2-hydroxyalkanoic acid + halide | - |
? | |
| monobromoacetate + H2O | - |
Burkholderia cepacia | glycolate + HBr | - |
? | |
| monochloroacetate + H2O | - |
Burkholderia cepacia | glycolate + HCl | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 27000, SDS-PAGE | Burkholderia cepacia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Burkholderia cepacia |
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