Application | Comment | Organism |
---|---|---|
synthesis | production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-mercuric chlorobenzoate | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | 28000 | - |
Pseudomonas sp. |
25000 | 28000 | - |
Rhizobium sp. |
25000 | 28000 | - |
Xanthobacter autotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-2-haloalkanoic acid + H2O | Rhizobium sp. | - |
L-2-hydroxyalkanoic acid + D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | Pseudomonas sp. | - |
D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | Rhizobium sp. | - |
D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | Xanthobacter autotrophicus | - |
D-2-hydroxyalkanoic acid + halide | - |
? | |
monochloroacetate + H2O | Pseudomonas sp. | - |
glycolate + HCl | - |
? | |
monochloroacetate + H2O | Rhizobium sp. | - |
glycolate + HCl | - |
? | |
monochloroacetate + H2O | Xanthobacter autotrophicus | - |
glycolate + HCl | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Rhizobium sp. | - |
- |
- |
Xanthobacter autotrophicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide | Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-haloacid + H2O | - |
Pseudomonas sp. | (R)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | - |
Rhizobium sp. | (R)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | - |
Xanthobacter autotrophicus | (R)-2-hydroxyacid + halide | - |
? | |
DL-2-haloalkanoic acid + H2O | - |
Rhizobium sp. | L-2-hydroxyalkanoic acid + D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-chloropropionic acid + H2O | - |
Pseudomonas sp. | D-2-hydroxypropionic acid + HCl | - |
? | |
L-2-haloalkanoic acid + H2O | - |
Pseudomonas sp. | D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | - |
Rhizobium sp. | D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | - |
Xanthobacter autotrophicus | D-2-hydroxyalkanoic acid + halide | - |
? | |
monochloroacetate + H2O | - |
Pseudomonas sp. | glycolate + HCl | - |
? | |
monochloroacetate + H2O | - |
Rhizobium sp. | glycolate + HCl | - |
? | |
monochloroacetate + H2O | - |
Xanthobacter autotrophicus | glycolate + HCl | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Pseudomonas sp. |