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Literature summary for 3.8.1.10 extracted from

  • Nardi-Dei, V.; Kurihara, T.; Park, C.; Esaki, N.; Soda, K.
    Bacterial DL-2-haloacid dehalogenase from Pseudomonas sp. strain 113: gene cloning and structural comparison with D- and L-2-haloacid dehalogenases (1997), J. Bacteriol., 179, 4232-4238.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, no significant sequence similarity between enzyme and L-2-haloacid dehalogenase is found, however, enzyme has significant sequence similarity with D-2-haloacid dehalogenase fom Pseudomonas putide AJ1 Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
additional information each of the 26 residues with charged and polar side chains, which are conserved between enzyme and D-2-haloacid dehalogenase, is mutated, Thr65, Glu69 and Asp194 are found to be essential Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide complete inactivation Pseudomonas sp.
D-2-chloropropionate inhibits dehalogenation of L-2-chloropropionate Pseudomonas sp.
diethyldicarbonate significant inactivation Pseudomonas sp.
L-2-chloropropionate inhibits dehalogenation of D-2-chloropropionate Pseudomonas sp.
N-bromosuccinimide significant inactivation Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
L-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.
4.2
-
D-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.
4.8
-
Monochloroacetate pH 9.5, 30°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34242
-
2 * 34242, calculated from amino acid sequence Pseudomonas sp.
68000
-
-
Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-2-haloacid + H2O Pseudomonas sp.
-
(S)-2-hydroxyacid + halide
-
?
(R)-2-haloacid + H2O Pseudomonas sp. 113
-
(S)-2-hydroxyacid + halide
-
?
(S)-2-haloacid + H2O Pseudomonas sp.
-
(R)-2-hydroxyacid + halide
-
?
(S)-2-haloacid + H2O Pseudomonas sp. 113
-
(R)-2-hydroxyacid + halide
-
?

Organism

Organism UniProt Comment Textmining
Achromobacter xylosoxidans Q59168
-
-
Pseudomonas putida
-
-
-
Pseudomonas putida AJ1
-
-
-
Pseudomonas sp. O06652
-
-
Pseudomonas sp. 113 O06652
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-haloacid + H2O
-
Pseudomonas putida (S)-2-hydroxyacid + halide
-
?
(R)-2-haloacid + H2O
-
Pseudomonas sp. (S)-2-hydroxyacid + halide
-
?
(R)-2-haloacid + H2O
-
Pseudomonas sp. 113 (S)-2-hydroxyacid + halide
-
?
(R)-2-haloacid + H2O
-
Pseudomonas putida AJ1 (S)-2-hydroxyacid + halide
-
?
(S)-2-haloacid + H2O
-
Pseudomonas putida (R)-2-hydroxyacid + halide
-
?
(S)-2-haloacid + H2O
-
Pseudomonas sp. (R)-2-hydroxyacid + halide
-
?
(S)-2-haloacid + H2O
-
Pseudomonas sp. 113 (R)-2-hydroxyacid + halide
-
?
(S)-2-haloacid + H2O
-
Pseudomonas putida AJ1 (R)-2-hydroxyacid + halide
-
?
D-2-chloropropionate + H2O
-
Pseudomonas sp. L-lactate + HCl
-
?
D-2-chloropropionate + H2O
-
Pseudomonas sp. 113 L-lactate + HCl
-
?
L-2-chloropropionate + H2O
-
Pseudomonas sp. D-lactate + HCl
-
?
L-2-chloropropionate + H2O
-
Pseudomonas sp. 113 D-lactate + HCl
-
?
monochloroacetate + H2O
-
Pseudomonas sp. glycolate + HCl
-
?
monochloroacetate + H2O
-
Pseudomonas sp. 113 glycolate + HCl
-
?

Subunits

Subunits Comment Organism
dimer 2 * 34242, calculated from amino acid sequence Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.2
-
D-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.
6.5
-
L-2-chloropropionate pH 9.5, 30°C Pseudomonas sp.