Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, no significant sequence similarity between enzyme and L-2-haloacid dehalogenase is found, however, enzyme has significant sequence similarity with D-2-haloacid dehalogenase fom Pseudomonas putide AJ1 | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | each of the 26 residues with charged and polar side chains, which are conserved between enzyme and D-2-haloacid dehalogenase, is mutated, Thr65, Glu69 and Asp194 are found to be essential | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | complete inactivation | Pseudomonas sp. | |
D-2-chloropropionate | inhibits dehalogenation of L-2-chloropropionate | Pseudomonas sp. | |
diethyldicarbonate | significant inactivation | Pseudomonas sp. | |
L-2-chloropropionate | inhibits dehalogenation of D-2-chloropropionate | Pseudomonas sp. | |
N-bromosuccinimide | significant inactivation | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
L-2-chloropropionate | pH 9.5, 30°C | Pseudomonas sp. | |
4.2 | - |
D-2-chloropropionate | pH 9.5, 30°C | Pseudomonas sp. | |
4.8 | - |
Monochloroacetate | pH 9.5, 30°C | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34242 | - |
2 * 34242, calculated from amino acid sequence | Pseudomonas sp. |
68000 | - |
- |
Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-haloacid + H2O | Pseudomonas sp. | - |
(S)-2-hydroxyacid + halide | - |
? | |
(R)-2-haloacid + H2O | Pseudomonas sp. 113 | - |
(S)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | Pseudomonas sp. | - |
(R)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | Pseudomonas sp. 113 | - |
(R)-2-hydroxyacid + halide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter xylosoxidans | Q59168 | - |
- |
Pseudomonas putida | - |
- |
- |
Pseudomonas putida AJ1 | - |
- |
- |
Pseudomonas sp. | O06652 | - |
- |
Pseudomonas sp. 113 | O06652 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-haloacid + H2O | - |
Pseudomonas putida | (S)-2-hydroxyacid + halide | - |
? | |
(R)-2-haloacid + H2O | - |
Pseudomonas sp. | (S)-2-hydroxyacid + halide | - |
? | |
(R)-2-haloacid + H2O | - |
Pseudomonas sp. 113 | (S)-2-hydroxyacid + halide | - |
? | |
(R)-2-haloacid + H2O | - |
Pseudomonas putida AJ1 | (S)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | - |
Pseudomonas putida | (R)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | - |
Pseudomonas sp. | (R)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | - |
Pseudomonas sp. 113 | (R)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | - |
Pseudomonas putida AJ1 | (R)-2-hydroxyacid + halide | - |
? | |
D-2-chloropropionate + H2O | - |
Pseudomonas sp. | L-lactate + HCl | - |
? | |
D-2-chloropropionate + H2O | - |
Pseudomonas sp. 113 | L-lactate + HCl | - |
? | |
L-2-chloropropionate + H2O | - |
Pseudomonas sp. | D-lactate + HCl | - |
? | |
L-2-chloropropionate + H2O | - |
Pseudomonas sp. 113 | D-lactate + HCl | - |
? | |
monochloroacetate + H2O | - |
Pseudomonas sp. | glycolate + HCl | - |
? | |
monochloroacetate + H2O | - |
Pseudomonas sp. 113 | glycolate + HCl | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34242, calculated from amino acid sequence | Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
D-2-chloropropionate | pH 9.5, 30°C | Pseudomonas sp. | |
6.5 | - |
L-2-chloropropionate | pH 9.5, 30°C | Pseudomonas sp. |