Literature summary for 3.7.1.8 extracted from

Jia, Y.; Wang, J.; Ren, C.; Nahurira, R.; Khokhar, I.; Wang, J.; Fan, S.; Yan, Y.
Identification and characterization of a meta-cleavage product hydrolase involved in biphenyl degradation from Arthrobacter sp. YC-RL1 (2019), Appl. Microbiol. Biotechnol., 103, 6825-6836 .

Search for more literature for 3.7.1.8

Application

Application	Comment	Organism
environmental protection	potential enzyme resource for the biodegradation of biphenyl, bioremediation of the environmental pollution caused by biphenyl/polychlorinated biphenyls	Arthrobacter sp. YC-RL1

Cloned(Commentary)

Cloned (Comment)	Organism
heterologously expressed in Escherichia coli BL21 (DE3)	Arthrobacter sp. YC-RL1

Protein Variants

Protein Variants	Comment	Organism
D247A	mutant enzyme retains 29.93% enzyme activity	Arthrobacter sp. YC-RL1
H275A	mutant enzyme retains 10.25% enzyme activity	Arthrobacter sp. YC-RL1
S115A	mutant enzyme retains 28.92% enzyme activity	Arthrobacter sp. YC-RL1

Inhibitors

Inhibitors	Comment	Organism	Structure
CHAPS	-	Arthrobacter sp. YC-RL1
DEPC	-	Arthrobacter sp. YC-RL1
DTT	-	Arthrobacter sp. YC-RL1
additional information	insensitive to EDTA	Arthrobacter sp. YC-RL1
NBS	-	Arthrobacter sp. YC-RL1
PMSF	-	Arthrobacter sp. YC-RL1
SDS	-	Arthrobacter sp. YC-RL1
Trition X-100	-	Arthrobacter sp. YC-RL1
Tween 20	-	Arthrobacter sp. YC-RL1
Tween 80	-	Arthrobacter sp. YC-RL1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.14	-	2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate	pH 7.0, 30°C	Arthrobacter sp. YC-RL1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O	Arthrobacter sp. YC-RL1	the enzyme is involved in the biodegradation of biphenyl	2-oxopent-4-enoate + salicylate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter sp. YC-RL1	A0A0H0ZQK0	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Arthrobacter sp. YC-RL1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O	-	Arthrobacter sp. YC-RL1	2-oxopent-4-enoate + salicylate	-	?
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O	the enzyme is involved in the biodegradation of biphenyl	Arthrobacter sp. YC-RL1	2-oxopent-4-enoate + salicylate	-	?

Synonyms

Synonyms	Comment	Organism
2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoate hydrolase	-	Arthrobacter sp. YC-RL1
BphD	-	Arthrobacter sp. YC-RL1
HOPDA hydrolase	-	Arthrobacter sp. YC-RL1

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	50	-	Arthrobacter sp. YC-RL1

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
15	55	15°C: about 45% of maximal activity, 55°C: about 70% of maximal activity	Arthrobacter sp. YC-RL1

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	1 h, the enzyme retains more than 60% residual activity	Arthrobacter sp. YC-RL1
60	-	1 h, the enzyme retains less than 60% residual activity	Arthrobacter sp. YC-RL1

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
11.61	-	2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate	pH 7.0, 30°C	Arthrobacter sp. YC-RL1

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Arthrobacter sp. YC-RL1

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	10	pH 5.0: about 65% of maximal activity, pH 9.0: about 85% of maximal activity, pH 10.0: about 35 % of maximal activity	Arthrobacter sp. YC-RL1

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	9	more than 60% of the activity is retained after incubation at pH 5.0-9.0 at 30°C for 10 min	Arthrobacter sp. YC-RL1

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the biodegradation of biphenyl	Arthrobacter sp. YC-RL1

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Release 2023.1 (January 2023)