Application | Comment | Organism |
---|---|---|
molecular biology | upstream of bphC are five ORFs, including bphD, exhibiting low homology with, and a different gene order from, previously characterized bph genes | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
expression from pBUPH2 in Escherichia coli MV1184 | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00071 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | at 60°C | Bacillus sp. (in: Bacteria) | |
0.00085 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | at 25°C | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) JF8 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, to homogeneity | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.34 | - |
at 60°C | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O | - |
Bacillus sp. (in: Bacteria) | benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid | - |
? | |
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O | - |
Bacillus sp. (in: Bacteria) JF8 | benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid | - |
? | |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
Bacillus sp. (in: Bacteria) | benzoate + 2-hydroxypenta-2,4-dienoate | - |
? | |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
Bacillus sp. (in: Bacteria) JF8 | benzoate + 2-hydroxypenta-2,4-dienoate | - |
? | |
additional information | faint activity towards 2-hydroxy-6-oxohepta-2,4-dienoate and 2-hydroxymuconic semialdehyde | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | faint activity towards 2-hydroxy-6-oxohepta-2,4-dienoate and 2-hydroxymuconic semialdehyde | Bacillus sp. (in: Bacteria) JF8 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
6-phenyl HODA hydrolase | - |
Bacillus sp. (in: Bacteria) |
BphD | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 85 | - |
Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.11 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | at 60°C | Bacillus sp. (in: Bacteria) |