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Literature summary for 3.7.1.8 extracted from

  • Mukerjee-Dhar, G.; Shimura, M.; Miyazawa, D.; Kimbara, K.; Hatta, T.
    bph genes of the thermophilic PCB degrader, Bacillus sp. JF8: characterization of the divergent ring-hydroxylating dioxygenase and hydrolase genes upstream of the Mn-dependent BphC (2005), Microbiology, 151, 4139-4151.
    View publication on PubMed

Application

Application Comment Organism
molecular biology upstream of bphC are five ORFs, including bphD, exhibiting low homology with, and a different gene order from, previously characterized bph genes Bacillus sp. (in: Bacteria)

Cloned(Commentary)

Cloned (Comment) Organism
expression from pBUPH2 in Escherichia coli MV1184 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00071
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate at 60°C Bacillus sp. (in: Bacteria)
0.00085
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate at 25°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) JF8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, to homogeneity Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.34
-
at 60°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
Bacillus sp. (in: Bacteria) benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
?
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
Bacillus sp. (in: Bacteria) JF8 benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
Bacillus sp. (in: Bacteria) benzoate + 2-hydroxypenta-2,4-dienoate
-
?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
Bacillus sp. (in: Bacteria) JF8 benzoate + 2-hydroxypenta-2,4-dienoate
-
?
additional information faint activity towards 2-hydroxy-6-oxohepta-2,4-dienoate and 2-hydroxymuconic semialdehyde Bacillus sp. (in: Bacteria) ?
-
?
additional information faint activity towards 2-hydroxy-6-oxohepta-2,4-dienoate and 2-hydroxymuconic semialdehyde Bacillus sp. (in: Bacteria) JF8 ?
-
?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
6-phenyl HODA hydrolase
-
Bacillus sp. (in: Bacteria)
BphD
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 85
-
Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate at 60°C Bacillus sp. (in: Bacteria)