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Literature summary for 3.7.1.7 extracted from

  • Shimao, M.; Tamogami, T.; Kishida, S.; Harayama, S.
    The gene pvaB encodes oxidized polyvinyl alcohol hydrolase of Pseudomonas sp. strain VM15C and forms an operon with the polyvinyl alcohol dehydrogenase gene pvaA (2000), Microbiology, 146, 649-657.
    View publication on PubMed
Search for more literature for 3.7.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
PMSF sugggesting that the enzyme is a serine hydrolase Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane specific activity in membrane fraction is several times higher than that of soluble fraction Pseudomonas sp. 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40610
-
 deduced from amino acid sequence Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 VM15C
-
Pseudomonas sp. VM15C
-
 VM15C
-

Reaction

Reaction Comment Organism Reaction ID
nonane-4,6-dione + H2O = pentan-2-one + butanoate putative serine hydrolase Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.33
-
 4,6-nonandione as substrate Pseudomonas sp.
1.65
-
 oxidized polyvinyl alcohol as substrate Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,6-nonanedione + H2O
-
 Pseudomonas sp. butyric acid + 2-pentanone
-
 ?
4,6-nonanedione + H2O
-
 Pseudomonas sp. VM15C butyric acid + 2-pentanone
-
 ?
polyvinyl alcohol + H2O
-
 Pseudomonas sp. carboxylic acid + methyl ketone
-
 ?
polyvinyl alcohol + H2O
-
 Pseudomonas sp. VM15C carboxylic acid + methyl ketone
-
 ?

Subunits

Subunits Comment Organism
monomer expressed in Escherichia coli Pseudomonas sp.