Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
the wild type enzyme is cocrystallized with 3-hydroxyhippuric acid, using the modified microbatch under oil technique at 25?C, with 0.05 M MgCl2, 0.1 M Tris (pH 8.0), and 25% (w/v) PEG 3000 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H102W/N333T | the mutant shows complete reversal of substrate specificity between 3-hydroxy-L-kynurenine and L-kynurenine | Homo sapiens |
H102W/S332G/N333T | the mutant shows complete reversal of substrate specificity between 3-hydroxy-L-kynurenine and L-kynurenine | Homo sapiens |
N333T | the mutant reveals a 9fold decrease in kcat for L-kynurenine, but no change in Km, the mutant has weaker 3-hydroxy-L-kyrenine binding (6fold higher Km) and kcat at least 1100times slower than wild type Kynase | Homo sapiens |
S332G/N333T the | mutant shows a very slight preference for L-kynurenine over 3-hydroxy-L-kynurenine | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-4-[3'-hydroxyphenyl]-4-hydroxybutanoic acid | most potent inhibitor | Homo sapiens | |
3-hydroxyhippuric acid | competitive inhibitor of kynureninase | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0283 | - |
3-hydroxy-L-kynurenine | wild type enzyme, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.2 | - |
3-hydroxy-L-kynurenine | Km above 0.2 mM, mutant enzyme N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.327 | - |
L-kynurenine | mutant enzyme H102W/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.495 | - |
L-kynurenine | wild type enzyme, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.499 | - |
L-kynurenine | mutant enzyme N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.51 | - |
L-kynurenine | Km above 0.51 mM, mutant enzyme H102W/S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.51 | - |
3-hydroxy-L-kynurenine | Km above 0.51 mM, mutant enzyme S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.54 | - |
L-kynurenine | mutant enzyme S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16719 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-L-kynurenine + H2O | preferred substrate | Homo sapiens | 3-hydroxyanthranilate + L-alanine | - |
? | |
L-kynurenine + H2O | - |
Homo sapiens | anthranilate + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Kynase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
3-hydroxy-L-kynurenine | kcat less than 0.0039 1/s, mutant enzyme N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.007 | - |
L-kynurenine | mutant enzyme H102W/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.0109 | - |
3-hydroxy-L-kynurenine | kcat less than 0.0109 1/s, mutant enzyme S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.017 | - |
L-kynurenine | mutant enzyme S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.018 | - |
L-kynurenine | Km above 0.51 mM, mutant enzyme H102W/S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.026 | - |
L-kynurenine | mutant enzyme N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
0.23 | - |
L-kynurenine | wild type enzyme, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens | |
3.5 | - |
3-hydroxy-L-kynurenine | wild type enzyme, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Homo sapiens |