Cloned (Comment) | Organism |
---|---|
into a pET100 plasmid for expression in Escherichia coli BL21DE3 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystals of recombinant kynureninase that diffracted to 2.0 A are obtained, and the atomic structure of the PLP-bound holoenzyme is determined by molecular replacement using the Pseudomonas fluorescens structure as the phasing model | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0283 | - |
3-hydroxy-DL-kynurenine | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-L-kynurenine + H2O | Homo sapiens | - |
3-hydroxyanthranilate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16719 | - |
- |
Purification (Comment) | Organism |
---|---|
using a Ni-CAM resin column | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.75 | - |
3-hydroxy-DL-kynurenine | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-DL-kynurenine + H2O | - |
Homo sapiens | 3-hydroxyanthranilate + DL-alanine | - |
? | |
3-hydroxy-L-kynurenine + H2O | - |
Homo sapiens | 3-hydroxyanthranilate + L-alanine | - |
? | |
L-kynurenine + H2O | - |
Homo sapiens | anthranilate + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
kynureninase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
3-hydroxy-DL-kynurenine | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
activity assay | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |