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Literature summary for 3.7.1.3 extracted from

  • Fitzgerald, D.H.; Muirhead, K.M.; Botting, N.P.
    A comparative study on the inhibition of human and bacterial kynureninase by novel bicyclic kynurenine analogues (2001), Bioorg. Med. Chem., 9, 983-989.
    View publication on PubMed
Search for more literature for 3.7.1.3

Application

Application Comment Organism
synthesis of inhibitors of kynureninase could prove to be useful in the development of the successful treatment regimen for neurological disorders such as septicemia, AIDS related dementia, Lyme disease, Huntington's and Alzheimer's disease Homo sapiens
synthesis of inhibitors of kynureninase could prove to be useful in the development of the successful treatment regimen for neurological disorders such as septicemia, AIDS related dementia, Lyme disease, Huntington's and Alzheimer's disease Pseudomonas fluorescens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-amino-(4-oxo-4-naphthyl)-butyric acid competitive inhibition, reversible inhibition by dilution Homo sapiens
2-amino-(4-oxo-4-naphthyl)-butyric acid competitive inhibition, reversible inhibition by dilution Pseudomonas fluorescens
amino-(1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-acetic acid competitive inhibition, reversible inhibition by dilution Homo sapiens
amino-(1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-acetic acid competitive inhibition, reversible inhibition by dilution Pseudomonas fluorescens
amino-(1-oxo-2,3-dihydro-1H-inden-2-yl)-acetic acid competitive inhibition, reversible inhibition by dilution Homo sapiens
amino-(1-oxo-2,3-dihydro-1H-inden-2-yl)-acetic acid competitive inhibition, reversible inhibition by dilution Pseudomonas fluorescens
amino-(4-oxo-3,4-dihydro-2H-chromen-3-yl)-acetic acid competitive inhibition, reversible inhibition by dilution Homo sapiens
amino-(4-oxo-3,4-dihydro-2H-chromen-3-yl)-acetic acid competitive inhibition, reversible inhibition by dilution Pseudomonas fluorescens
beta-chloro-L-alanine irreversible inhibition Homo sapiens
beta-chloro-L-alanine irreversible inhibition Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0442
-
 L-kynurenine at 37ºC, pH 7.5 Pseudomonas fluorescens
0.0442
-
 3-hydroxy-L-kynurenine at 37ºC, pH 7.5 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxy-L-kynurenine + H2O Homo sapiens biosynthesis of NAD+ 3-hydroxyanthranilate + L-alanine
-
 ?
3-hydroxy-L-kynurenine + H2O Pseudomonas fluorescens biosynthesis of NAD+ 3-hydroxyanthranilate + L-alanine
-
 ?
L-kynurenine + H2O Homo sapiens L-tryptophan metabolism anthranilate + L-alanine
-
 ?
L-kynurenine + H2O Pseudomonas fluorescens L-tryptophan metabolism anthranilate + L-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Pseudomonas fluorescens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
 Homo sapiens
no modification
-
 Pseudomonas fluorescens

Purification (Commentary)

Purification (Comment) Organism
by protamine sulfate precipitation, ammonium sulfate precipitation, DEAE-Sepharose 6B column, Sephadex G-100 column, Mono-Q HR 5/5 column, 50-80fold Pseudomonas fluorescens

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Pseudomonas fluorescens
-
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.008 0.015 pH 7.5, 37ºC Homo sapiens
1.2 2.2 pH 7.5, 37ºC Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-L-kynurenine + H2O
-
 Homo sapiens 3-hydroxyanthranilate + L-alanine
-
 ?
3-hydroxy-L-kynurenine + H2O
-
 Pseudomonas fluorescens 3-hydroxyanthranilate + L-alanine
-
 ?
3-hydroxy-L-kynurenine + H2O biosynthesis of NAD+ Homo sapiens 3-hydroxyanthranilate + L-alanine
-
 ?
3-hydroxy-L-kynurenine + H2O biosynthesis of NAD+ Pseudomonas fluorescens 3-hydroxyanthranilate + L-alanine
-
 ?
L-kynurenine + H2O
-
 Homo sapiens anthranilate + L-alanine
-
 ?
L-kynurenine + H2O
-
 Pseudomonas fluorescens anthranilate + L-alanine
-
 ?
L-kynurenine + H2O L-tryptophan metabolism Homo sapiens anthranilate + L-alanine
-
 ?
L-kynurenine + H2O L-tryptophan metabolism Pseudomonas fluorescens anthranilate + L-alanine
-
 ?

Synonyms

Synonyms Comment Organism
L-kynurenine hydrolase
-
 Homo sapiens
L-kynurenine hydrolase
-
 Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Homo sapiens
pyridoxal 5'-phosphate
-
 Pseudomonas fluorescens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.005
-
 2-amino-(4-oxo-4-naphthyl)-butyric acid at 37ºC, pH 7.5 Pseudomonas fluorescens
0.022
-
 2-amino-(4-oxo-4-naphthyl)-butyric acid at 37ºC, pH 7.5 Homo sapiens
0.0349
-
 amino-(1-oxo-2,3-dihydro-1H-inden-2-yl)-acetic acid at 37ºC, pH 7.5 Pseudomonas fluorescens
0.045
-
 amino-(1-oxo-2,3-dihydro-1H-inden-2-yl)-acetic acid at 37ºC, pH 7.5 Homo sapiens
0.077
-
 amino-(4-oxo-3,4-dihydro-2H-chromen-3-yl)-acetic acid at 37ºC, pH 7.5 Homo sapiens
0.162
-
 amino-(4-oxo-3,4-dihydro-2H-chromen-3-yl)-acetic acid at 37ºC, pH 7.5 Pseudomonas fluorescens
0.17
-
 amino-(1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-acetic acid at 37ºC, pH 7.5 Pseudomonas fluorescens
0.227
-
 amino-(1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-acetic acid at 37ºC, pH 7.5 Homo sapiens