Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas fluorescens |
into vector pTZKYN and transformed in Escherichia coli XL-1 Blue cells | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S)-2-amino-4-oxo-4-phenylbutanoic acid | competitive inhibitor of the reaction of L-kynurenine with the enzyme | Pseudomonas fluorescens | |
beta-benzoyl-L-alanine | competitive inhibition, at 25ºC, pH 7.8 | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
beta-benzoyl-L-alanine | at 25ºC, pH 7.8 | Pseudomonas fluorescens | |
0.03 | - |
L-kynurenine | at 25ºC, pH 7.8 | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + H2O | Pseudomonas fluorescens | L-tryptophan metabolism | anthranilate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no modification | - |
Pseudomonas fluorescens |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
by chromatography on phenyl-Sepharose | Pseudomonas fluorescens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Pseudomonas fluorescens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
19 U/mg protein | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-amino-4-oxo-4-phenylbutanoic acid + H2O | the rate-determining step is Cbeta-Cgamma bond cleavage | Pseudomonas fluorescens | benzoate + L-alanine | - |
? | |
beta-benzoyl-L-alanine + H2O | - |
Pseudomonas fluorescens | benzoate + L-alanine | - |
? | |
L-kynurenine + H2O | - |
Pseudomonas fluorescens | anthranilate + L-alanine | - |
? | |
L-kynurenine + H2O | L-tryptophan metabolism | Pseudomonas fluorescens | anthranilate + L-alanine | - |
? | |
L-kynurenine + H2O | the rate-determining step is to be deprotonation of the pyruvate-ketimine intermediate | Pseudomonas fluorescens | anthranilate + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-kynurenine hydrolase | - |
Pseudomonas fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
beta-benzoyl-L-alanine | - |
Pseudomonas fluorescens | |
0.7 | - |
(2S)-2-amino-4-oxo-4-phenylbutanoic acid | at pH 7.8 | Pseudomonas fluorescens | |
16 | - |
L-kynurenine | - |
Pseudomonas fluorescens | |
16 | - |
L-kynurenine | at pH 7.8 | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas fluorescens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
beta-benzoyl-L-alanine | at 25ºC, pH 7.8 | Pseudomonas fluorescens | |
0.01 | - |
(2S)-2-amino-4-oxo-4-phenylbutanoic acid | reaction of L-kynurenine with the enzyme | Pseudomonas fluorescens |