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Literature summary for 3.7.1.25 extracted from

  • Riddle, R.; Gibbs, P.; Willson, R.; Benedik, M.
    Purification and properties of 2-hydroxy-6-oxo-6(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase involved in microbial degradation of carbazole (2003), Protein Expr. Purif., 28, 182-189 .
    View publication on PubMed
Search for more literature for 3.7.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0046
-
 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate at pH 7.0 and 60°C Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. LD2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal affinity column chromatography Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate + H2O
-
 Pseudomonas sp. 2-hydroxypenta-2,4-dienoate + benzoate
-
 ?
2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate + H2O
-
 Pseudomonas sp. LD2 2-hydroxypenta-2,4-dienoate + benzoate
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Pseudomonas sp.

Synonyms

Synonyms Comment Organism
2-hydroxy-6-oxo-6(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase
-
 Pseudomonas sp.
HOPDA hydrolase
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10.5 the enzyme exhibits activity between pH 6.5 and 10.5 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme has no cofactor requirement Pseudomonas sp.