Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas alcaligenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | complete inhibition at 1 mM | Pseudomonas alcaligenes | |
Co2+ | complete inhibition at 1 mM | Pseudomonas alcaligenes | |
Cu2+ | complete inhibition at 1 mM | Pseudomonas alcaligenes | |
Ni2+ | complete inhibition at 1 mM | Pseudomonas alcaligenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0199 | - |
3-maleylpyruvate | at pH 7.6 and 30°C | Pseudomonas alcaligenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | addition of Mn2+ at 0.05 or 0.1 mM enhances the enzyme activity by 30% | Pseudomonas alcaligenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, SDS-PAGE | Pseudomonas alcaligenes |
77000 | - |
gel filtration | Pseudomonas alcaligenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-maleylpyruvate + H2O | Pseudomonas alcaligenes | - |
maleate + pyruvate | - |
? | |
3-maleylpyruvate + H2O | Pseudomonas alcaligenes NCIMB 9867 | - |
maleate + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas alcaligenes | Q0QFQ3 | - |
- |
Pseudomonas alcaligenes NCIMB 9867 | Q0QFQ3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose column chromatography | Pseudomonas alcaligenes |
Storage Stability | Organism |
---|---|
-80°C, with 50% (v/v) glycerol, 20 days, 38% loss of activity | Pseudomonas alcaligenes |
-80°C, with 50% (v/v) glycerol, 50 days, 73% loss of activity | Pseudomonas alcaligenes |
4°C, with 5% (v/v) glycerol, 10 days, 50% loss of activity | Pseudomonas alcaligenes |
4°C, with 5% (v/v) glycerol, 40 days, 79% loss of activity | Pseudomonas alcaligenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-maleylpyruvate + H2O | - |
Pseudomonas alcaligenes | maleate + pyruvate | - |
? | |
3-maleylpyruvate + H2O | - |
Pseudomonas alcaligenes NCIMB 9867 | maleate + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 33000, SDS-PAGE | Pseudomonas alcaligenes |
Synonyms | Comment | Organism |
---|---|---|
hbzF | - |
Pseudomonas alcaligenes |
maleylpyruvate hydrolase | - |
Pseudomonas alcaligenes |
Mph | - |
Pseudomonas alcaligenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas alcaligenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Pseudomonas alcaligenes |