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Literature summary for 3.7.1.2 extracted from
- Cloning and expression of fumarylacetoacetate hydrolase derived from marine yeast Rhodosporidium diobovatum (2015), J. Basic Microbiol., 55, 1082-1093.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of FAH genomic DNA and cDNA by degenerate PCR and RACE method, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3) from pET28a-FAH | Rhodotorula diobovata |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
4-Fumarylacetoacetate | pH 7.3, 25°C | Rhodotorula diobovata |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, SDS-PAGE | Rhodotorula diobovata |
| 66000 | - |
about, native PAGE | Rhodotorula diobovata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-fumarylacetoacetate + H2O | Rhodotorula diobovata | - |
acetoacetate + fumarate | - |
? | |
| 4-fumarylacetoacetate + H2O | Rhodotorula diobovata MCCC 2A00023 | - |
acetoacetate + fumarate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula diobovata | A0A076VF18 | - |
- |
| Rhodotorula diobovata MCCC 2A00023 | A0A076VF18 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 5.0fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and anion exchange chromatography | Rhodotorula diobovata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.77 | - |
purified recombinant enzyme, substrate fumarylacetoacetate, pH 7.3, 25°C | Rhodotorula diobovata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-fumarylacetoacetate + H2O | - |
Rhodotorula diobovata | acetoacetate + fumarate | - |
? | |
| 4-fumarylacetoacetate + H2O | - |
Rhodotorula diobovata MCCC 2A00023 | acetoacetate + fumarate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 33000, SDS-PAGE | Rhodotorula diobovata |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAH | - |
Rhodotorula diobovata |
| fumarylacetoacetate hydrolase | - |
Rhodotorula diobovata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rhodotorula diobovata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
assay at | Rhodotorula diobovata |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rhodotorula diobovata | isoelectric focusing | 6.1 | 5.9 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | fumarylacetoacetate hydrolase is a metabolic enzyme that participates in phenylalanine and tyrosine catabolism and can catalyze the hydrolytic cleavage of carbon-carbon bonds in a variety of diketo acid substrates. Fumarylacetoacetate hydrolase catalyzes the hydrolytic cleavage of a carbon-carbon bond in fumarylacetoacetate to yield acetoacetate and fumarate as the final step in tyrosine degradation | Rhodotorula diobovata |
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Release 2023.1 (January 2023)
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