BRENDA - Enzyme Database
show all sequences of 3.7.1.2

The purification and properties of a triacetic acid-hydrolyzing enzyme

Connors, W.M.; Stotz, E.; J. Biol. Chem. 178, 881-890 (1949)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Benzoylacetone
9 mM
Bos taurus
citrate
9 mM
Bos taurus
Fe3+
inactivation by precipitating the proteins
Bos taurus
La3+
inactivation by precipitating the proteins
Bos taurus
malonate
9 mM
Bos taurus
oxalate
9 mM
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.38
-
triacetic acid
-
Bos taurus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
-
Bos taurus
Mg2+
-
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Storage Stability
Storage Stability
Organism
4C, 1 month
Bos taurus
frozen, 3 months
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4-diketohexanoate + H2O
-
289239
Bos taurus
pyruvate + propanoate
-
289239
Bos taurus
?
triacetic acid + H2O
-
289239
Bos taurus
acetoacetic acid + acetic acid
-
289239
Bos taurus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
in maleate buffer
Bos taurus
8
-
in phosphate and borate buffer
Bos taurus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8.5
-
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Benzoylacetone
9 mM
Bos taurus
citrate
9 mM
Bos taurus
Fe3+
inactivation by precipitating the proteins
Bos taurus
La3+
inactivation by precipitating the proteins
Bos taurus
malonate
9 mM
Bos taurus
oxalate
9 mM
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.38
-
triacetic acid
-
Bos taurus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
-
Bos taurus
Mg2+
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Storage Stability (protein specific)
Storage Stability
Organism
4C, 1 month
Bos taurus
frozen, 3 months
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4-diketohexanoate + H2O
-
289239
Bos taurus
pyruvate + propanoate
-
289239
Bos taurus
?
triacetic acid + H2O
-
289239
Bos taurus
acetoacetic acid + acetic acid
-
289239
Bos taurus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
in maleate buffer
Bos taurus
8
-
in phosphate and borate buffer
Bos taurus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8.5
-
Bos taurus
Other publictions for EC 3.7.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734103
Liu
Cloning and expression of fuma ...
Rhodotorula diobovata, Rhodotorula diobovata MCCC 2A00023
J. Basic Microbiol.
55
1082-1093
2015
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1
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1
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1
1
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734967
Han
Disruption of fumarylacetoacet ...
Arabidopsis thaliana
Plant Physiol.
162
1956-1964
2013
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1
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4
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2
2
-
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699824
Werner
Intestinal epithelial cell pro ...
Mus musculus
J. Proteome Res.
8
3252-3264
2009
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1
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-
706809
Battilana
The 1-deoxy-D: -xylulose 5-pho ...
Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis vinifera
Theor. Appl. Genet.
118
653-669
2009
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2
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-
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-
-
2
2
-
-
-
686266
Kang
Characterization of a homogent ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis O6
Curr. Microbiol.
56
145-149
2008
-
-
-
-
-
-
-
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2
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687809
Fisher
The Caenorhabditis elegans K10 ...
Caenorhabditis elegans
J. Biol. Chem.
283
9127-9135
2008
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1
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1
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1
1
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5
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1
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1
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1
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1
1
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1
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-
-
-
-
-
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-
-
-
-
697797
Eggenhofer
Allogeneic bone marrow transpl ...
Mus musculus
Exp. Hematol.
36
1507-1513
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
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1
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-
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-
-
-
-
-
-
-
-
684183
Mizutani
Purification, crystallization ...
Thermus thermophilus
Acta Crystallogr. Sect. F
63
792-794
2007
-
1
1
1
-
-
-
-
-
-
1
1
-
2
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1
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1
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1
1
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1
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1
1
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1
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-
1
-
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-
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-
-
-
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-
-
-
-
684964
Bateman
Slow-onset inhibition of fumar ...
Homo sapiens, Mus musculus
Biochem. J.
402
251-260
2007
-
2
2
1
-
-
4
-
-
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2
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5
-
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2
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1
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2
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2
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2
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2
2
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1
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4
2
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2
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2
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1
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2
-
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-
2
-
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-
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-
670622
Dixon
Enzymes of tyrosine catabolism ...
Arabidopsis thaliana
Plant Sci.
171
360-366
2006
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1
1
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1
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4
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1
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1
1
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1
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2
1
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657866
Grogan
Emergent mechanistic diversity ...
Bos taurus, Mus musculus
Biochem. J.
388
721-730
2005
-
-
-
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2
1
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5
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1
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3
1
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1
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1
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3
1
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-
669086
Arias-Barrau
The homogentisate pathway: a c ...
Pseudomonas putida, Pseudomonas putida U-dhmgB
J. Bacteriol.
186
5062-5077
2004
-
1
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4
-
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2
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-
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-
-
-
-
670957
Williams
D-serine-induced nephrotoxicit ...
Rattus norvegicus
Toxicology
201
231-238
2004
-
-
-
-
-
-
1
-
-
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3
-
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2
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1
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1
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-
655997
Bateman
Mechanistic inferences from th ...
Homo sapiens, Mus musculus
J. Biol. Chem.
276
15284-15291
2001
-
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2
1
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2
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5
-
2
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3
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2
2
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4
-
2
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1
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2
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1
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2
1
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5
-
2
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2
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4
-
2
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289257
Timm
Crystal structure and mechanis ...
Mus musculus
Structure
7
1023-1033
1999
-
1
-
1
13
-
-
-
-
1
1
1
-
2
-
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1
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1
1
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1
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1
13
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1
1
1
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1
1
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289256
Awata
Structural organization and an ...
Homo sapiens
Biochim. Biophys. Acta
1226
168-172
1994
-
1
1
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1
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2
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1
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1
1
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1
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1
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-
289252
Kvittingen
Hereditary tyrosinemia type I ...
Homo sapiens
J. Clin. Invest.
91
1816-1821
1993
-
1
-
-
-
-
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1
-
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2
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3
1
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1
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1
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1
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3
1
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1
-
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-
-
-
-
289253
Labelle
Localization of cells in the r ...
Homo sapiens, Rattus norvegicus
Biochim. Biophys. Acta
1180
250-256
1993
-
2
-
-
-
-
-
-
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1
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4
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7
-
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2
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1
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7
-
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289254
Grompe
Loss of fumarylacetoacetate hy ...
Mus musculus
Genes Dev.
7
2298-2307
1993
-
1
-
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3
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2
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289255
Labelle
Characterization of the human ...
Homo sapiens
Hum. Mol. Genet.
2
941-946
1993
-
1
1
-
1
-
-
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-
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2
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1
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1
1
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1
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1
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289251
Klebig
Murine fumarylacetoacetate hyd ...
Mus musculus
Proc. Natl. Acad. Sci. USA
89
1363-1367
1992
-
1
1
-
-
-
-
-
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3
-
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2
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1
1
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-
-
2
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-
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-
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-
-
-
-
-
289249
Phaneuf
Cloning and expression of the ...
Homo sapiens
Am. J. Hum. Genet.
48
525-535
1991
-
1
1
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-
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1
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2
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3
-
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1
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1
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1
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1
1
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-
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1
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2
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1
-
1
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-
1
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289250
Labelle
Cloning and expression analysi ...
Rattus norvegicus
Gene
104
197-202
1991
-
1
1
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
4
-
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1
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-
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-
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1
1
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1
-
-
-
-
1
-
4
-
-
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1
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Van Faassen
Purification of human liver fu ...
Homo sapiens
J. Biochem. Biophys. Methods
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1990
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289248
Laberge
Fumarylacetoacetase measuremen ...
Homo sapiens
Am. J. Hum. Genet.
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325-328
1990
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289246
Nagainis
Effects of pH and sulfhydryl s ...
Bos taurus
Biochim. Biophys. Acta
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203-211
1981
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289243
Mahuran
Characterization and substrate ...
Bos taurus
Can. J. Biochem.
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1-8
1977
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289244
Lindblad
On the enzymic defects in here ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
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4641-4645
1977
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289245
Doshi
Localization of fumarylacetoac ...
Rattus norvegicus
Can. J. Biochem.
56
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1977
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289242
Braun
Inhibition of fumarylacetoacet ...
Bos taurus
Biochemistry
12
4878-4881
1973
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17
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289241
Hsiang
Purification and properties of ...
Bos taurus
Biochemistry
11
2098-2102
1972
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289240
Edwards
Homogentisate metabolism: The ...
Bos taurus, Rattus norvegicus
J. Biol. Chem.
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1956
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289239
Connors
The purification and propertie ...
Bos taurus
J. Biol. Chem.
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881-890
1949
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289238
Meister
Enzymatic hydrolysis of 2,4-di ...
Felis catus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
175
573-588
1948
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28
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