BRENDA - Enzyme Database
show all sequences of 3.7.1.19

An alpha/beta-fold C-C bond hydrolase is involved in a central step of nicotine catabolism by Arthrobacter nicotinovorans

Sachelaru, P.; Schiltz, E.; Igloi, G.L.; Brandsch, R.; J. Bacteriol. 187, 8516-8519 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli XL-1 Blue cells
Paenarthrobacter nicotinovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
2,6-dihydroxypseudooxynicotine
in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration
Paenarthrobacter nicotinovorans
43555
-
1 * 43555, calculated from amino acid sequence
Paenarthrobacter nicotinovorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paenarthrobacter nicotinovorans
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-chelating Sepharose column chromatography
Paenarthrobacter nicotinovorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,6-dihydroxypseudooxynicotine + H2O
-
719704
Paenarthrobacter nicotinovorans
2,6-dihydroxypyridine + 4-methylaminobutanoate
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 43000, SDS-PAGE; 1 * 43555, calculated from amino acid sequence
Paenarthrobacter nicotinovorans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
792
-
2,6-dihydroxypseudooxynicotine
in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication
Paenarthrobacter nicotinovorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Paenarthrobacter nicotinovorans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
10
-
Paenarthrobacter nicotinovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli XL-1 Blue cells
Paenarthrobacter nicotinovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
2,6-dihydroxypseudooxynicotine
in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration
Paenarthrobacter nicotinovorans
43555
-
1 * 43555, calculated from amino acid sequence
Paenarthrobacter nicotinovorans
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-chelating Sepharose column chromatography
Paenarthrobacter nicotinovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,6-dihydroxypseudooxynicotine + H2O
-
719704
Paenarthrobacter nicotinovorans
2,6-dihydroxypyridine + 4-methylaminobutanoate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 43000, SDS-PAGE; 1 * 43555, calculated from amino acid sequence
Paenarthrobacter nicotinovorans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
792
-
2,6-dihydroxypseudooxynicotine
in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication
Paenarthrobacter nicotinovorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Paenarthrobacter nicotinovorans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
10
-
Paenarthrobacter nicotinovorans
Other publictions for EC 3.7.1.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
684760
Ganas
Two closely related pathways o ...
Nocardioides sp., Nocardioides sp. JS614 / ATCC BAA-499, Paenarthrobacter nicotinovorans
Arch. Microbiol.
189
511-517
2008
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3
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3
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719704
Sachelaru
An alpha/beta-fold C-C bond hy ...
Paenarthrobacter nicotinovorans
J. Bacteriol.
187
8516-8519
2005
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1
1
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1
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1
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2
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1
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1
1
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1
1
1
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