Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method. The 2.1 A resolution X-ray structure of the native enzyme determined from orthorhombic crystals confirms that it is a member of the alpha/beta hydrolase fold family, comprising eight beta-strands interconnected by loops and helices. The 2.8 A resolution structure of the enzyme cocrystallized with the non-hydrolysable substrate analogue 2,6-diketo-nona-1,9-dioic acid confirms the location of the active site in a buried channel including Ser110, His263 and Asp235, postulated contributors to a serine protease-like catalytic triad in homologous enzymes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-diketo-nona-1,9-dioic acid | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
2 * 31000 | Escherichia coli |
62000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O | Escherichia coli | the enzyme is involved in 3-phenylpropanoate catabolism | (2E)-2-hydroxypenta-2,4-dienoate + succinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77044 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O | the enzyme is involved in 3-phenylpropanoate catabolism | Escherichia coli | (2E)-2-hydroxypenta-2,4-dienoate + succinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 31000 | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase | - |
Escherichia coli |
MhpC | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in 3-phenylpropanoate catabolism | Escherichia coli |