Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H114A | shows twofold-reduced catalytic efficiency, ruling out a catalytic role, but shows a fivefold-reduced KM for the natural substrate, and an ability to process an aryl-containing substrate, implying a role for His114 in positioning of the substrate | Escherichia coli |
H263A | mutant exhibits very slow ketonisation and C-C cleavage | Escherichia coli |
S110A | exhibits fast ketonisation, an intermediate phase, and slow C-C cleavage | Escherichia coli |
S110G | exhibits fast ketonisation, an intermediate phase, and slow C-C cleavage | Escherichia coli |
S40A | shows twofold reduced catalytic efficiency, but shows a very fast interconversion of dienol to dienolate forms of the substrate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme S40A | Escherichia coli | |
0.0025 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme H114A | Escherichia coli | |
0.0055 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme H263A | Escherichia coli | |
0.0068 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, wild-type enzyme | Escherichia coli | |
0.0175 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme S110G | Escherichia coli | |
0.0185 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme S110A | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O | Escherichia coli | the enzyme is involved in 3-phenylpropanoate catabolism | (2E)-2-hydroxypenta-2,4-dienoate + succinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77044 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + succinate | a catalytic mechanism is proposed involving stabilisation of reactive intermediates and activation of a nucleophilic water molecule by Ser110 | Escherichia coli | |
(2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + fumarate | a catalytic mechanism is proposed involving stabilisation of reactive intermediates and activation of a nucleophilic water molecule by Ser110 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O | - |
Escherichia coli | (2E)-2-hydroxypenta-2,4-dienoate + succinate | - |
? | |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O | the enzyme is involved in 3-phenylpropanoate catabolism | Escherichia coli | (2E)-2-hydroxypenta-2,4-dienoate + succinate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxy-6-keto-nona-1,9-dioic acid 5,6-hydrolase | - |
Escherichia coli |
MhpC | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme S110G | Escherichia coli | |
0.0029 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme H263A | Escherichia coli | |
0.0054 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme S110A | Escherichia coli | |
0.016 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme D235A, approximate value from activity of the cell extract | Escherichia coli | |
2 | 8 | (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, wild-type enzyme | Escherichia coli | |
2.9 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme S40A | Escherichia coli | |
4.5 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme H114A | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in 3-phenylpropanoate catabolism | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme S110G | Escherichia coli | |
0.29 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme S110A | Escherichia coli | |
5.27 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme H263A | Escherichia coli | |
242 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme S40A | Escherichia coli | |
412 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, wild-type enzyme | Escherichia coli | |
1800 | - |
(2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate | pH 8.0, 25°C, mutant enzyme H114A | Escherichia coli |