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Literature summary for 3.6.5.6 extracted from
- Insight into the GTPase activity of tubulin from complexes with stathmin-like domains (2007), Biochemistry, 46, 10595-10602.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Colchicine | - |
Bos taurus |  |
| stathmin-like domains | - |
Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-terminal part of stathimin like-domains | - |
Bos taurus | |
| vinblastine | - |
Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Bos taurus | - |
GDP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| bovine brain tubulin is purified by two cycles of polymerization and depolymerization | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 50 mM Mes-K buffer, pH 6.8, 33% glycerol, 0.25 mM MgCl2, 0.5 mM EGTA, 0.1 mM GTP | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Bos taurus | GDP + phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | alpha-beta heterodimer | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tubulin GTPase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
GTPase activity assay | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000037 | - |
GTP | tubulin, ligand podophylotoxin | Bos taurus | |
| 0.000053 | - |
GTP | tubulin in complex with T2R, ligand podophylotoxin | Bos taurus | |
| 0.000055 | - |
GTP | tubulin | Bos taurus | |
| 0.000056 | - |
GTP | tubulin in complex with T2R | Bos taurus | |
| 0.000125 | - |
GTP | tubulin, ligand colchicine | Bos taurus | |
| 0.000258 | - |
GTP | tubulin in complex with T2R, ligand colchicine | Bos taurus | |
| 0.00037 | - |
GTP | tubulin in complex with Rhe1, ligand colchicine | Bos taurus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
GTPase activity assay | Bos taurus |
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Release 2023.1 (January 2023)
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