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Literature summary for 3.6.5.5 extracted from

  • Lomash, R.M.; Gu, X.; Youle, R.J.; Lu, W.; Roche, K.W.
    Neurolastin, a dynamin family GTPase, regulates excitatory synapses and spine density (2015), Cell Rep., 12, 743-751.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.5

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of HA-tagged neurolastin in HEK cells, recombinant expression of GST-tagged wild-type and mutant enzymes in HeLa cells Mus musculus

Protein Variants

Protein Variants Comment Organism
C85A site-directed mutagenesis, a dominant-negative mutant Mus musculus
H97W site-directed mutagenesis, a RING mutant Mus musculus
additional information construction of neurolastin knockout mice Mus musculus
R340Q site-directed mutagenesis, GTPase inactive mutant Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
endocytic vesicle neurolastin is peripherally associated with membranes via its C-terminus and localizes to endocytic vesicles Mus musculus 30139
-
endosome
-
 Mus musculus 5768
-
membrane neurolastin is peripherally associated with membranes via its C-terminus but seems to contains two transmembrane segments Mus musculus 16020
-
synapse
-
 Mus musculus 45202
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mus musculus
Zn2+ binding coordinates are conserved Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Mus musculus
-
 GDP + phosphate
-
 ?
additional information Mus musculus in addition to the GTPase domain, neurolastin also has an E3 ubiquitin ligase domain, the enzyme shows E3 ligase activity in an in vitro ubiquitination assay ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
hippocampus
-
 Mus musculus
-
neuron
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Mus musculus GDP + phosphate
-
 ?
additional information in addition to the GTPase domain, neurolastin also has an E3 ubiquitin ligase domain, the enzyme shows E3 ligase activity in an in vitro ubiquitination assay Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
dynamin family GTPase
-
 Mus musculus
neurolastin
-
 Mus musculus

General Information

General Information Comment Organism
evolution neurolastin is a unique dynamin family GTPase with a RING domain, sequence analysis shows that neurolastin has a C3HC4-type RING domain and key residues important for zinc coordination are conserved Mus musculus
physiological function dynamin family GTPases are key membrane remodeling mechanoenzymes. Neurolastin, a dynamin family GTPase, regulates excitatory synapses and spine density. Neurolastin affects endosome size via its RING domain. It is important for excitatory neurotransmission Mus musculus