Cloned (Comment) | Organism |
---|---|
recombinant expression of the GST-tagged chimeric fusion protein of AtDRP1A fusion protein in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
purified recombinant GST-tagged chimeric fusion protein of dynamin-related protein 1A, AtDRP1A, comprising GTPase domain of Arabidopsis thaliana AtDRP1A fused to its GTPase effector domain (GED) in a nucleotide-associated form, hanging drop vapour diffusion method, mixing of 5 mg/ml dimeric protein in 20 mM Tris-HCl, pH 8.0, 150 mM NaC, with 160 mM sodium formate, 16% w/v PEG 3350, 40 mM magnesium chloride, 6% w/v 2-propanol, 20 mM HEPES, pH 7.5, method optimization, X-ray diffraction structure determination and analysis at 3.6 A resolution, molecular replacement using the crystal structure of human dynamin GG dimer, PDB ID 2X2E | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of an AtDRP1A fusion protein consisting of residues 1-316 of the GTPase domain and residues 585-606 of GED fused by a flexible linker with the primary sequence HGTDSRV as suggested from human dynamin | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
dimer of dimers, 4 * 39000, crystal structure analysis | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Arabidopsis thaliana | - |
GDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P42697 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged chimeric fusion protein of AtDRP1A fusion protein from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Arabidopsis thaliana | GDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | dimer of dimers, 4 * 39000, crystal structure analysis | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtDRP1A | - |
Arabidopsis thaliana |
dynamin-related protein 1A | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme AtDRP1A belongss to the DRP1 dynamin-related subfamily of the superfamily of GTPases. Dynamins and other structurally related family members primarily consist of a GTPase domain, a middle domain and a GTPase effector domain, which regulates and responds to hydrolysis of the GTPase domain. The GED and the Nand C-termini of the GTPase domain form a so-called three-helical bundle signalling element that regulates dynamin function | Arabidopsis thaliana |
metabolism | plant-specific dynamin-related proteins play crucial roles in cell-plate formation, endocytosis or exocytosis, protein sorting to the vacuole and plasma membrane and the division of mitochondria and chloroplasts | Arabidopsis thaliana |
physiological function | interaction between the enzyme's GTPase domain and its GED domain is important for regulating dynamin function | Arabidopsis thaliana |