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Literature summary for 3.6.5.5 extracted from

  • Klinglmayr, E.; Wenger, J.; Mayr, S.; Bossy-Wetzel, E.; Puehringer, S.
    Purification, crystallization and X-ray diffraction analysis of human dynamin-related protein 1 GTPase-GED fusion protein (2012), Acta Crystallogr. Sect. F, 68, 1217-1221.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.5

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of nucleotide-free GTPase-GTPase effector domain fusion protein of enzyme Drp1, and wild-type and mutant full-length enzymes in Escherichia coli strain BL21(DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant dynamin-related protein 1 GTPase-GTPase effector domain fusion protein, sitting drop vapour diffusion method, mixing of 0.0004 ml of protein solution and reservoir solution, the latter containing 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 5.5, 25% v/v PEG 3350 or 0.1 M sodium citrate, pH 5.5, 20% PEG 3000, 20°C, X-ray diffraction structure determination and analysis at 2.67 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
K38A site-directed mutagenesis, enzymatically inactive mutant Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state GTPase activities of recombinant Drp1 GTPase-GTPase effector fusion protein Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40923
-
 x * 40923, nucleotide-free GTPase-GTPase effector domain fusion protein of Drp1, mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O00429
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant nucleotide-free GTPase-GTPase effector domain fusion protein of human Drp1, and wild-type and mutant full-length enzymes from Escherichia coli strain BL21(DE3) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ?
GTP + H2O the recombinant GTPase-GTPase effector domain fusion protein of human Drp1 shows GTPase activity Homo sapiens GDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 40923, nucleotide-free GTPase-GTPase effector domain fusion protein of Drp1, mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
Drp1
-
 Homo sapiens
dynamin-related protein 1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction dysregulation of enzyme Drp1 is associated with abnormal mitochondrial dynamics and neuronal damage Homo sapiens
metabolism the enzyme is a key regulator of mitochondrial fission Homo sapiens
additional information enzyme Drp1 shares structural and functional similarities with dynamin 1 with respect to domain organization, ability to self-assemble into spiral-like oligomers and GTP-cycle-dependent membrane scission. The enzyme has an additional unstructured region between the middle domain and GTPase effector domain Homo sapiens
physiological function the mechano-enzyme dynamin-related protein 1 plays an important role in mitochondrial fission and is implicated in cell physiology Homo sapiens