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Literature summary for 3.6.5.5 extracted from

  • Xi, Q.; Pauer, G.J.; Ball, S.L.; Rayborn, M.; Hollyfield, J.G.; Peachey, N.S.; Crabb, J.W.; Hagstrom, S.A.
    Interaction between the photoreceptor-specific tubby-like protein 1 and the neuronal-specific GTPase dynamin-1 (2007), Invest. Ophthalmol. Vis. Sci., 48, 2837-2844.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine dynamin-1 interacts with TULP1, TULP1 is a photoreceptor-specific protein that is mutated in retinitis pigmentosa Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
the full-length mouse dynamin-1 cDNA isolated from retina is subcloned into the pGEX-2TK vector, the construct is used to generate three different dynamin-1 domain constructs, the N-terminal domain, aa 1-520, the proline-rich domain, aa 750-814, and the C-terminal domain, aa 521-814 Mus musculus

Protein Variants

Protein Variants Comment Organism
GST-Dyn1-C single domain of dynamin-1, constructed for the identification of the interaction domains between dynamin-1 and TULP1 Mus musculus
GST-Dyn1-N single domain of dynamin-1, constructed for the identification of the interaction domains between dynamin-1 and TULP1 Mus musculus
GST-Dyn1-PRD single domain of dynamin-1, constructed for the identification of the interaction domains between dynamin-1 and TULP1 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
identified in immunoprecipitation experiments by MS/MS sequence analysis Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Mus musculus
-
GDP + phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione S-transferase dynamin-1 fusion constructs are expressed in Escherichia coli BL21 cells, the proteins are purified using glutathione-Sepharose 4B beads Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
outer plexiform layer
-
Mus musculus
-
photoreceptor photoreceptor cell Mus musculus
-
retina
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Mus musculus GDP + phosphate
-
ir

Synonyms

Synonyms Comment Organism
dynamin GTPase
-
Mus musculus
dynamin-1
-
Mus musculus
GTPase dynamin-1
-
Mus musculus