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Literature summary for 3.6.5.5 extracted from

  • Tanaka, A.; Kobayashi, S.; Fujiki, Y.
    Peroxisome division is impaired in a CHO cell mutant with an inactivating point-mutation in dynamin-like protein 1 gene (2006), Exp. Cell Res., 312, 1671-1684.
    View publication on PubMed
Search for more literature for 3.6.5.5

Cloned(Commentary)

Cloned (Comment) Organism
a CHO-K1 cDNA library in the pSPORT vector is screened, the PCR product, derived from a positive clone, is cloned using the vectors pcDNA3.1Zeo+Myc6-, pcDNA3.1Zeo+Flag-, pcDNA3.1Zeo+HA2-, and pGEX6P-2, DLP1 cDNA from ZP121 is subcloned into pGEM-Teasy Cricetulus griseus

Protein Variants

Protein Variants Comment Organism
G363D mutant shows dominant-negative activity to normal DLP1, mutation causes lipid transport deficiency Cricetulus griseus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Cricetulus griseus 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Cricetulus griseus
-
 GDP + phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DLP1 and its variants are expressed as glutathione-S-transferase-fusion proteins in Escherichia coli and are purified with glutathione-Sepharose beads Cricetulus griseus

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell
-
 Cricetulus griseus
-
CHO-K1 cell
-
 Cricetulus griseus
-
ZP121 cell CHO cell line Cricetulus griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Cricetulus griseus GDP + phosphate
-
 ir

Synonyms

Synonyms Comment Organism
DLP1
-
 Cricetulus griseus
dynamin-like protein 1
-
 Cricetulus griseus