Literature summary for 3.6.5.5 extracted from

Damke, H.; Baba, T.; Warnock, D.E.; Schmid, S.L.
Induction of mutant dynamin specifically blocks endocytic coated vesicle formation (1994), J. Cell. Biol., 127, 915-934.

Search for more literature for 3.6.5.5

Activating Compound

Activating Compound	Comment	Organism	Structure
microtubule	activates GTPase activity	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and K44A mutant dynamin-1 in HeLa cells and in Sf9 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
K44A	mutant defective in GTP binding and hydrolysis, mutation in the first of three nucleotide binding elements, in HeLa cells overexpressing mutant dynamin, coated pits fail to become constricted and coated vesicles fail to bud, so that endocytosis via both transferrin and EGF receptors is potently inhibited, these cells accumulate long tubules, many of which remain connected to the plasma membrane, detailed biochemical and morphological analysis of the functional consequences of overexpression of the dynamin mutant	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
clathrin-coated vesicle	dynamin is associated with isolated coated vesicles	Homo sapiens	30136	-
cytosol	cytosolic protein, dynamin undergoes a cycle of membrane association/dissociation coupled to the cycle of GTP exchange and hydrolysis	Homo sapiens	5829	-
plasma membrane	membrane-bound dynamin is specifically targeted to clathrin-coated pits at the plasma membrane, where it is required for coated pit constriction and coated vesicle budding, dynamin undergoes a cycle of membrane association/dissociation coupled to the cycle of GTP exchange and hydrolysis	Homo sapiens	5886	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
100000	-	x * 100000, about, Western blot analysis	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Homo sapiens	dynamin plays a role in clathrin-mediated endocytosis, it plays a role in vesicle budding, dynamin is specifically required for endocytic coated vesicle formation and its GTP binding and hydrolysis activities are required to form constricted coated pits and, subsequently, for coated vesicle budding	GDP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	neuron-specific isoform: dynamin-1	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and K44A mutant dynamin-1, expressed in Sf9 cells	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
neuron	neuron-specific dynamin-1	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Homo sapiens	GDP + phosphate	-	?
GTP + H2O	dynamin plays a role in clathrin-mediated endocytosis, it plays a role in vesicle budding, dynamin is specifically required for endocytic coated vesicle formation and its GTP binding and hydrolysis activities are required to form constricted coated pits and, subsequently, for coated vesicle budding	Homo sapiens	GDP + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 100000, about, Western blot analysis	Homo sapiens

Synonyms

Synonyms	Comment	Organism
dynamin-1	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)