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Literature summary for 3.6.5.5 extracted from

  • Song, B.D.; Leonard, M.; Schmid, S.L.
    Dynamin GTPase domain mutants that differentially affect GTP binding, GTP hydrolysis, and clathrin-mediated endocytosis (2004), J. Biol. Chem., 279, 40431-40436.
    View publication on PubMed
Search for more literature for 3.6.5.5

Activating Compound

Activating Compound Comment Organism Structure
additional information dynamin can self-assemble forming higher order structures such as rings and spirals that exhibit up to 100fold stimulated GTPase activity, conformational changes in the active site accompany self-assembly Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene for dynamin1, expression in HEK293-cre4 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information effect of dynamin GTPase domain mutations on GTP binding, GTP hydrolysis and clathrin-mediated endocytosis Homo sapiens
S61A mutation lowers the rate of assembly-stimulated GTP hydrolysis without altering the Km for GTP, kinetic data Homo sapiens
S61D kinetic data Homo sapiens
T141A mutation increases the rate of assembly-stimulated GTP hydrolysis without altering the Km for GTP, kinetic data Homo sapiens
T141D kinetic data Homo sapiens
T65A mutation dramatically lowers both the rate of assembly-stimulated GTP hydrolysis and the affinity for GTP, kinetic data Homo sapiens
T65D mutation dramatically lowers both the rate of assembly-stimulated GTP hydrolysis and the affinity for GTP, kinetic data Homo sapiens
T65H mutation dramatically lowers both the rate of assembly-stimulated GTP hydrolysis and the affinity for GTP, kinetic data Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data, temperature-dependent effects on kinetic parameters, self-assembly alters the Km for GTP Homo sapiens
0.0034
-
 GTP 22°C, wild-type dynamin, basal GTPase activity Homo sapiens
0.0053
-
 GTP 22°C, wild-type dynamin, lipid tubule stimulated GTPase activity Homo sapiens
0.037
-
 GTP 37°C, wild-type dynamin, lipid tubule stimulated GTPase activity Homo sapiens
0.041
-
 GTP 37°C, S61A mutant dynamin: lipid tubule stimulated GTPase activity, T65D mutant dynamin: basal GTPase activity Homo sapiens
0.056
-
 GTP 37°C, S61D and T141A mutant dynamin, lipid tubule stimulated GTPase activity Homo sapiens
0.067
-
 GTP 37°C, S61A mutant dynamin, basal GTPase activity Homo sapiens
0.068
-
 GTP 37°C, S61D mutant dynamin, basal GTPase activity Homo sapiens
0.102
-
 GTP 37°C, wild-type dynamin, basal GTPase activity Homo sapiens
0.149
-
 GTP 37°C, T65H mutant dynamin, basal GTPase activity Homo sapiens
0.174
-
 GTP 37°C, T141A mutant dynamin, basal GTPase activity Homo sapiens
0.513
-
 GTP 37°C, T65D mutant dynamin, lipid tubule stimulated GTPase activity Homo sapiens
0.763
-
 GTP 37°C, T141D mutant dynamin, basal GTPase activity Homo sapiens
0.88
-
 GTP 37°C, T65A mutant dynamin, basal GTPase activity Homo sapiens
0.988
-
 GTP 37°C, T65H mutant dynamin, lipid tubule stimulated GTPase activity Homo sapiens
1.94
-
 GTP 37°C, T141D mutant dynamin, lipid tubule stimulated GTPase activity Homo sapiens
2.115
-
 GTP 37°C, T65A mutant dynamin, lipid tubule stimulated GTPase activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens GTP hydrolysis is required for clathrin-mediated endocytosis in vivo GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 recombinant dynamin1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant dynamin-1 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O GTP hydrolysis is required for clathrin-mediated endocytosis in vivo Homo sapiens GDP + phosphate
-
 ?
GTP + H2O conformational changes in the active site accompany self-assembly, dynamin domain structure, Thr-65 plays an important role in catalysis, Thr-141 has roles in both GTP binding and hydrolysis Homo sapiens GDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
More dynamin can self-assemble forming higher order structures such as rings and spirals that exhibit up to 100fold stimulated GTPase activity Homo sapiens

Synonyms

Synonyms Comment Organism
dynamin 1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 37 both the basal and assembly-stimulated rates of GTP hydrolysis for wild-type dynamin are 10fold lower when assayed at 22°C compared with 37°C, effect of these temperatures on the Km Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data, temperature-dependent effects on kinetic parameters Homo sapiens
0.0003
-
 GTP 37°C, T65D and T65H mutant dynamin, basal GTPase activity Homo sapiens
0.0013
-
 GTP 37°C, T65A mutant dynamin, basal GTPase activity Homo sapiens
0.0025
-
 GTP 37°C, T141D mutant dynamin, basal GTPase activity Homo sapiens
0.0032
-
 GTP 22°C, wild-type dynamin, basal GTPase activity Homo sapiens
0.0072
-
 GTP 37°C, S61D mutant dynamin, basal GTPase activity Homo sapiens
0.016
-
 GTP 37°C, T65D and T65H mutant dynamin, lipid tubule stimulated GTPase activity Homo sapiens
0.02
-
 GTP 37°C, T65A mutant dynamin, lipid tubule stimulated GTPase activity Homo sapiens
0.025
-
 GTP 37°C, T141D mutant dynamin, lipid tubule stimulated GTPase activity Homo sapiens
0.032
-
 GTP 37°C, S61A mutant dynamin, basal GTPase activity Homo sapiens
0.043
-
 GTP 37°C, wild-type dynamin, basal GTPase activity Homo sapiens
0.112
-
 GTP 37°C, T141A mutant dynamin, basal GTPase activity Homo sapiens
0.13
-
 GTP 22°C, wild-type dynamin, lipid tubule stimulated GTPase activity Homo sapiens
0.44
-
 GTP 37°C, S61D mutant dynamin, lipid tubule stimulated GTPase activity Homo sapiens
1.43
-
 GTP 37°C, S61A mutant dynamin, lipid tubule stimulated GTPase activity Homo sapiens
1.75
-
 GTP 37°C, wild-type dynamin, lipid tubule stimulated GTPase activity Homo sapiens
3.08
-
 GTP 37°C, T141A mutant dynamin, lipid tubule stimulated GTPase activity Homo sapiens