Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-Rosetta2 cells | Pyrococcus furiosus |
Crystallization (Comment) | Organism |
---|---|
free and GDP-magnesium-bound forms, hanging drop vapour diffusion method, the hexagonal form grows in 1.1-1.5 M ammonium phosphate and 100 mM sodium acetate pH 5.0. The monoclinic form grows in 0.9-1.2 M lithium sulfate, 0.4-0.6 M ammonium sulfate, and 100 mM sodium citrate pH 5.0. For the GDP-bound structure, best crystals grow in 14-17% (w/v) PEG 8000 and 100 mM Tris pH 8.0 | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34900 | - |
apo-enzyme, analytical ultracentrifugation and small-angle X-ray scattering | Pyrococcus furiosus |
35810 | - |
apo-enzyme, calculated from amino acid sequence | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U051 | - |
- |
Purification (Comment) | Organism |
---|---|
heat selective precipitation, cobalt-chelating affinity chromatography, gel filtration, and ion-exchange chromatography | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Pyrococcus furiosus | GDP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FtsY | - |
Pyrococcus furiosus |
signal recognition particle receptor | - |
Pyrococcus furiosus |
SRP | - |
Pyrococcus furiosus |