Cloned (Comment) | Organism |
---|---|
the vector pJGS3 expressing the protein FtsY NGd20, in which the N-terminal residues 2-20 of FtsY are deleted, is constructed by PCR-based site-directed mutagenesis of pTB88 for expression in Escherichia coli Rosetta-2DE3 pLysS cells | Thermus aquaticus |
Crystallization (Comment) | Organism |
---|---|
the 2.1 A X-ray structure of FtsY from Thermus aquaticus bound to GDP is reported | Thermus aquaticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Thermus aquaticus | - |
GDP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus aquaticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the FtsY NGd20 protein is purified over a HiTrap Blue column, desalted, then passed over a HiTrap SP and a HiTrap Q Sepharose column | Thermus aquaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Thermus aquaticus | GDP + phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
heterodimer | FtsY and Ffh assemble in a GTP-dependent manner to form a heterodimeric SRP targeting complex | Thermus aquaticus |
Synonyms | Comment | Organism |
---|---|---|
FtsY | GTPase receptor, comprises a GTPase domain similar to Ffh | Thermus aquaticus |
signal-recognition-particle GTPase | - |
Thermus aquaticus |
SRP GTPase | - |
Thermus aquaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
GTPase assay | Thermus aquaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
GTPase assay | Thermus aquaticus |