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Literature summary for 3.6.5.4 extracted from

  • Dong, H.J.; Tao, S.M.; Li, Y.Q.; Chan, S.H.; Shen, X.L.; Wang, C.X.; Guan, W.J.
    Analysis of the GTPase activity and active sites of the NG domains of FtsY and Ffh from Streptomyces coelicolor (2006), Acta Biochim. Biophys. Sin. (Shanghai), 38, 467-476.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the NG domain of Ffh is cloned into pUCm-T, and into pET-15b for expression in Escherichia coli BL21 cells Streptomyces coelicolor
the NG domain of FtsY is cloned into pUCm-T, and into pET-15b for expression in Escherichia coli BL21 cells Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
A145S substitution introduced into the putative GTP binding motif GXXGXGK Streptomyces coelicolor
G141V substitution introduced into the putative GTP binding motif GXXGXGK Streptomyces coelicolor
G222V substitution introduced into the putative GTP binding motif GXXGXGK Streptomyces coelicolor
GAK-VSG substitution introduced into the putative GTP binding motif GXXGXGK Streptomyces coelicolor
GTK-VSG substitution introduced into the putative GTP binding motif GXXGXGK Streptomyces coelicolor
K147G substitution introduced into the putative GTP binding motif GXXGXGK Streptomyces coelicolor
K228G substitution introduced into the putative GTP binding motif GXXGXGK Streptomyces coelicolor
T226S substitution introduced into the putative GTP binding motif GXXGXGK Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00081
-
GTP NG-Ffh, wild-type Streptomyces coelicolor
0.001131
-
GTP NG-Ffh, mutant A145S Streptomyces coelicolor
0.001143
-
GTP NG-FtsY, wild-type Streptomyces coelicolor
0.001183
-
GTP NG-FtsY, mutant T226S Streptomyces coelicolor
0.001265
-
GTP NG-Ffh, mutant G141V Streptomyces coelicolor
0.002879
-
GTP NG-FtsY, mutant K228G Streptomyces coelicolor
0.00349
-
GTP NG-FtsY, mutant GTK-VSG Streptomyces coelicolor
0.00493
-
GTP NG-FtsY, mutant G222V Streptomyces coelicolor
0.005075
-
GTP NG-Ffh, mutant K147G Streptomyces coelicolor
0.005128
-
GTP NG-Ffh, mutant GAK-VSG Streptomyces coelicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Streptomyces coelicolor 5737
-
membrane
-
Streptomyces coelicolor 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
NG domain of Ffh, determined by SDS-PAGE Streptomyces coelicolor
36000
-
NG domain of FtsY, determined by SDS-PAGE Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Streptomyces coelicolor
-
GDP + phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
proteins are purified using histidine-binding magnetic agarose beads Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Streptomyces coelicolor GDP + phosphate
-
ir

Synonyms

Synonyms Comment Organism
Ffh
-
Streptomyces coelicolor
FtsY
-
Streptomyces coelicolor
signal-recognition-particle GTPase
-
Streptomyces coelicolor
SRP GTPase
-
Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
GTPase activity assay Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
GTPase activity assay Streptomyces coelicolor