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Literature summary for 3.6.5.4 extracted from

  • Peluso, P.; Shan, S.O.; Nock, S.; Herschlag, D.; Walter, P.
    Role of SRP RNA in the GTPase cycles of Ffh and FtsY (2001), Biochemistry, 40, 15224-15233.
    View publication on PubMed
Search for more literature for 3.6.5.4

Activating Compound

Activating Compound Comment Organism Structure
4.5S RNA accelerates the association between Ffh and FtsY 400fold in their GTP-bound form and thereby stimulates GTPase activity Escherichia coli
FtsY GTPase subunit of the bacterial SRP receptor, both Ffh and FtsY act as GTPase activating proteins for one another, resulting in a mutual stimulation of GTP hydrolysis by both proteins Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
GDP
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic and thermodynamic data Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Escherichia coli cotranslational protein targeting to the plasma membrane, the Ffh-4.5S RNA ribonucleoprotein complex and the FtsY protein, respectively, form a unique complex in which both Ffh and FtsY act as GTPase activating proteins for one another, resulting in a mutual stimulation of GTP hydrolysis by both proteins, 4.5S RNA modulates the conformation of the Ffh-FtsY complex and may regulate its GTPase activity during the SRP functional cycle GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
GTP + H2O = GDP + phosphate kinetic and thermodynamic data Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O cotranslational protein targeting to the plasma membrane, the Ffh-4.5S RNA ribonucleoprotein complex and the FtsY protein, respectively, form a unique complex in which both Ffh and FtsY act as GTPase activating proteins for one another, resulting in a mutual stimulation of GTP hydrolysis by both proteins, 4.5S RNA modulates the conformation of the Ffh-FtsY complex and may regulate its GTPase activity during the SRP functional cycle Escherichia coli GDP + phosphate
-
 ?
GTP + H2O GTPase reaction cycle Escherichia coli GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Ffh
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
 GDP pH 7.5, 25°C, in the absence of 4.5S RNA Escherichia coli
0.00032
-
 GDP pH 7.5, 25°C, in the presence of 4.5S RNA Escherichia coli