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Literature summary for 3.6.5.3 extracted from

  • Sprink, T.; Ramrath, D.J.; Yamamoto, H.; Yamamoto, K.; Loerke, J.; Ismer, J.; Hildebrand, P.W.; Scheerer, P.; Buerger, J.; Mielke, T.; Spahn, C.M.
    Structures of ribosome-bound initiation factor 2 reveal the mechanism of subunit association (2016), Sci. Adv., 2, e1501502 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged IF2 in Escherichia coli using the T5 expression system Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
ribosome
-
Escherichia coli 5840
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Escherichia coli
-
GDP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A705
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged IF2 from Escherichia coli by affinity chromatography and ion exchange chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Escherichia coli GDP + phosphate
-
?
additional information formation of the 70S-fMet-tRNAi Met-IF2-GDPNP complex. 70S ribosomes are isolated from Escherichia coli strain CAN20, recombinant His-tagged IF2 enzyme, non-hydrolyzable GTP analogue GDPNP Escherichia coli ?
-
-

Synonyms

Synonyms Comment Organism
IF-2
-
Escherichia coli
IF2
-
Escherichia coli
initiation factor 2
-
Escherichia coli
ribosome-bound initiation factor 2
-
Escherichia coli
translational guanosine triphosphatase
-
Escherichia coli
trGTPase
-
Escherichia coli

General Information

General Information Comment Organism
additional information high-resolution structure for the key initiation trGTPase, initiation factor 2 (IF2), complexed with a nonhydrolyzable guanosine triphosphate analogue and initiator fMet-tRNAi Met in the context of the Escherichia coli ribosome to 3.7 A resolution using cryo-electron microscopy. Analysis of the intrinsic conformational modes of the 70S initiation complex (IC), establishing the mutual interplay of IF2 and initator transfer RNA (tRNA) with the ribsosome, mechanism of the final steps of translation initiation, overview. IF2-induced subunit joining of the 30S IC with the 50S subunit occurs in a rotated conformation and leads to the formation of the 70S-IC I. The initiator tRNA is positioned in the P/ei state through interactions with the L1 stalk and domain IV of IF2. Partial back rotation and unswiveling facilitate the P/pi state of initiator tRNA and reorient the G-domain of IF2 to trigger GTP hydrolysis. To reach the elongation-competent 70S complex, the 30S subunit completes back rotation, IF2-GDP dissociates, and the initiator tRNA completes the partial reverse translocation on the 50S subunit to reach the P/P-site state Escherichia coli