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Literature summary for 3.6.5.3 extracted from

  • Luviano, A.; Cruz-Castaneda, R.; Sanchez-Puig, N.; Garcia-Hernandez, E.
    Cooperative energetic effects elicited by the yeast Shwachman-Diamond syndrome protein (Sdo1) and guanine nucleotides modulate the complex conformational landscape of the elongation factor-like 1 (Efl1) GTPase (2019), Biophys. Chem., 247, 13-24 .
    View publication on PubMed
Search for more literature for 3.6.5.3

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae strain BCY123 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information isothermal titration calorimetry and kinetics at 20°C, binding energetics of Efl1 to guanine nucleotides, overview Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
ribosome
-
 Homo sapiens 5840
-
ribosome
-
 Saccharomyces cerevisiae 5840
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Saccharomyces cerevisiae
-
 GDP + phosphate
-
 ?
GTP + H2O Saccharomyces cerevisiae ATCC 204508
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q7Z2Z2
-
-
Saccharomyces cerevisiae P53893
-
-
Saccharomyces cerevisiae ATCC 204508 P53893
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Saccharomyces cerevisiae strain BCY123 by nickel affinity chromatography, tag cleavage by TEV protease, and another step of nickel affinity chromatography, followed by gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Saccharomyces cerevisiae GDP + phosphate
-
 ?
GTP + H2O
-
 Saccharomyces cerevisiae ATCC 204508 GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Efl1
-
 Homo sapiens
Efl1
-
 Saccharomyces cerevisiae
Efl1 GTPase
-
 Homo sapiens
Efl1 GTPase
-
 Saccharomyces cerevisiae
elongation factor-like 1
-
 Homo sapiens
elongation factor-like 1
-
 Saccharomyces cerevisiae
elongation factor-like 1 GTPase
-
 Homo sapiens
elongation factor-like 1 GTPase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 about, melting point, recombinant enzyme Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
additional information calorimetric energetic basis describing the recognition of Efl1 to GT(D)P, Sdo1 and their intercommunication in solution, overview. The structure based analysis of the binding signatures indicates that Efl1 has a large structural flexibility Saccharomyces cerevisiae
physiological function one of the final maturation steps of the large ribosomal subunit requires the joint action of the elongation factor-like 1 (EFL1) GTPase and the Shwachman-Diamond syndrome protein (SBDS) to release the eukaryotic translation initiation factor 6 (eIF6) and allow the assembly of mature ribosomes. EFL1 function is driven by conformational changes Homo sapiens
physiological function one of the final maturation steps of the large ribosomal subunit requires the joint action of the elongation factor-like 1 (Efl1) GTPase and the Shwachman-Diamond syndrome protein (SDO1, UniProt ID Q07953) to release the eukaryotic translation initiation factor 6 (Tif6) and allow the assembly of mature ribosomes. EFL1 function is driven by conformational changes Saccharomyces cerevisiae