Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.5.3 extracted from

  • Holtkamp, W.; Wintermeyer, W.; Rodnina, M.V.
    Synchronous tRNA movements during translocation on the ribosome are orchestrated by elongation factor G and GTP hydrolysis (2014), Bioessays, 36, 908-918.
    View publication on PubMed
Search for more literature for 3.6.5.3

Protein Variants

Protein Variants Comment Organism
H91A a GTPase-defective EF-G mutant Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Fusidic acid
-
 Escherichia coli
Fusidic acid
-
 Thermus thermophilus
additional information in free enzyme EF-G, crucial sensors of switch I and II regions are disordered and become ordered in the complex with a nonhydrolyzable GTP analogue, GDPCP, on the ribosome. This causes a reorientation of EF-G such that the tip of domain IV moves and the CHI state ofthe ribosome is stabilized Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Thermus thermophilus
-
 GDP + phosphate
-
 ?
GTP + H2O Escherichia coli
-
 GDP + phosphate
-
 ?
additional information Escherichia coli EF-G binding, without GTP hydrolysis, promotes slow and possibly incomplete translocation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Thermus thermophilus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
GTP + H2O = GDP + phosphate reaction mechanism, overview Thermus thermophilus
a
GTP + H2O = GDP + phosphate reaction mechanism, overview Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Thermus thermophilus GDP + phosphate
-
 ?
GTP + H2O
-
 Escherichia coli GDP + phosphate
-
 ?
additional information EF-G binding, without GTP hydrolysis, promotes slow and possibly incomplete translocation Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
EF-G
-
 Escherichia coli
EF-Tu
-
 Thermus thermophilus
elongation factor G
-
 Thermus thermophilus
elongation factor G
-
 Escherichia coli

General Information

General Information Comment Organism
additional information overall scheme of translocation: in the pre-translocation state, deacylated tRNA is bound in the P site and peptidy-tRNAl in the A site, both bound to their cognate codons in the mRNA. Following the binding of EF-G-GTP to the pretranslocation complex, translocation takes place. In the post-translocation state, peptidyl-tRNA has moved to the P site, whereas deacylated tRNA has dissociated, as have inorganic phosphate and EF-G-GDP. A histidine residue in the switch II region, His84 in Thermus thermophilus EF-G, plays an essential role in the reaction, structure-function relationships, overview Thermus thermophilus
additional information overall scheme of translocation: in the pre-translocation state, deacylated tRNA is bound in the P site and peptidy-tRNAl in the A site, both bound to their cognate codons in the mRNA. Following the binding of EF-G-GTP to the pretranslocation complex, translocation takes place. In the post-translocation state, peptidyl-tRNA has moved to the P site, whereas deacylated tRNA has dissociated, as have inorganic phosphate and EF-G-GDP. A histidine residue in the switch II region, His91 in Escherichia coli EF-G, plays an essential role in the reaction, structure-function relationships, overview Escherichia coli
physiological function synchronous tRNA movements during translocation on the ribosome are orchestrated by elongation factor G and GTP hydrolysis Thermus thermophilus
physiological function synchronous tRNA movements during translocation on the ribosome are orchestrated by elongation factor G and GTP hydrolysis Escherichia coli