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Literature summary for 3.6.5.3 extracted from

  • Wu, H.; Sun, L.; Blombach, F.; Brouns, S.J.; Snijders, A.P.; Lorenzen, K.; van den Heuvel, R.H.; Heck, A.J.; Fu, S.; Li, X.; Zhang, X.C.; Rao, Z.; van der Oost, J.
    Structure of the ribosome associating GTPase HflX. (2010), Proteins, 78, 705-713.
    View publication on PubMed
Search for more literature for 3.6.5.3

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of HflX from Sulfolobus solfataricus solved to 2.0 A resolution in apo- and GDP-bound forms Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
additional information the N-terminal deletion mutant displays a similar Km value as the wild-type enzyme whereas the substrate kcat is 24fold increased Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0129
-
 GTP pH 7.8, 50°C, N-terminal deletion mutant Saccharolobus solfataricus
0.0141
-
 GTP pH 7.8, 50°C, full-length enzyme Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q980M3
-
-
Saccharolobus solfataricus P2 Q980M3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O slow GTPase with relatively low affinity for GTP Saccharolobus solfataricus GDP + phosphate
-
 ?
GTP + H2O slow GTPase with relatively low affinity for GTP Saccharolobus solfataricus P2 GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
GTPase HflX
-
 Saccharolobus solfataricus
SSO0269 locus name Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00105
-
 GTP pH 7.8, 50°C, full-length enzyme Saccharolobus solfataricus
0.026
-
 GTP pH 7.8, 50°C, N-terminal deletion mutant Saccharolobus solfataricus