Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pulvomycin | increasing pulvomycin concentration increased the rate of the intrinsic GTPase catalysed by elongation factor 1alpha, reaching its maximum stimulation effect at 30 mM. Pulvomycin exerts its stimulatory function at all the tested temperatures (45-75°C). | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
conformational change of the elongation factor takes place upon interaction with the antibiotic. Protection against chemical denaturation of SsEF-1alpha is observed in the presence of pulvomycin | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | GTPase activity in the presence of a molar concentration of NaCl is competitively inhibited | Saccharolobus solfataricus | |
guanosine-5'-[(beta,gamma)-imido]triphosphate | i.e. GppNHp. GTPase activity in the presence of a molar concentration of NaCl is competitively inhibited | Saccharolobus solfataricus | |
pulvomycin | the antibiotic is able to reduce in vitro the rate of protein synthesis however, the concentration of pulvomycin leading to 50% inhibition (173 mM) is two order of magnitude higher but one order lower than that required in eubacteria and eukarya, respectively. Pulvomycin is able to decrease the affinity of the elongation factor toward aa-tRNA only in the presence of GTP, to an extent similar to that measured in the presence of GDP | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00436 | - |
GTP | pH 7.8, 50°C, GTPase activity in the presence 3.6 M NaCl | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
guanidine-HCl | 50% inactivation of GTPase activity at 3.0 M guanidine-HCl in presence 3.6 M NaCl and absence of pulvomycin. 50% inactivation of GTPase activity at 3.6 M guanidine-HCl in presence 3.6 M NaCl and presence of pulvomycin | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Saccharolobus solfataricus | GDP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
elongation factor 1alpha | - |
Saccharolobus solfataricus |
SsEF-1alpha | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
GTP | pH 7.8, 50°C, GTPase activity in the presence 3.6 M NaCl | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
GDP | pH 7.8, 50°C, GTPase activity in the presence 3.6 M NaCl | Saccharolobus solfataricus | |
0.00047 | - |
guanosine-5'-[(beta,gamma)-imido]triphosphate | pH 7.8, 50°C, GTPase activity in the presence 3.6 M NaCl | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.69 | - |
GTP | pH 7.8, 50°C, GTPase activity in the presence 3.6 M NaCl | Saccharolobus solfataricus |