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Literature summary for 3.6.5.3 extracted from

  • Martucci, N.M.; Lamberti, A.; Arcari, P.; Masullo, M.
    The eubacterial protein synthesis inhibitor pulvomycin interacts with archaeal elongation factor 1alpha from Sulfolobus solfataricus (2012), Biochimie, 94, 503-509.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
pulvomycin increasing pulvomycin concentration increased the rate of the intrinsic GTPase catalysed by elongation factor 1alpha, reaching its maximum stimulation effect at 30 mM. Pulvomycin exerts its stimulatory function at all the tested temperatures (45-75°C). Saccharolobus solfataricus

General Stability

General Stability Organism
conformational change of the elongation factor takes place upon interaction with the antibiotic. Protection against chemical denaturation of SsEF-1alpha is observed in the presence of pulvomycin Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
GDP GTPase activity in the presence of a molar concentration of NaCl is competitively inhibited Saccharolobus solfataricus
guanosine-5'-[(beta,gamma)-imido]triphosphate i.e. GppNHp. GTPase activity in the presence of a molar concentration of NaCl is competitively inhibited Saccharolobus solfataricus
pulvomycin the antibiotic is able to reduce in vitro the rate of protein synthesis however, the concentration of pulvomycin leading to 50% inhibition (173 mM) is two order of magnitude higher but one order lower than that required in eubacteria and eukarya, respectively. Pulvomycin is able to decrease the affinity of the elongation factor toward aa-tRNA only in the presence of GTP, to an extent similar to that measured in the presence of GDP Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00436
-
GTP pH 7.8, 50°C, GTPase activity in the presence 3.6 M NaCl Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent Comment Organism
guanidine-HCl 50% inactivation of GTPase activity at 3.0 M guanidine-HCl in presence 3.6 M NaCl and absence of pulvomycin. 50% inactivation of GTPase activity at 3.6 M guanidine-HCl in presence 3.6 M NaCl and presence of pulvomycin Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Saccharolobus solfataricus GDP + phosphate
-
?

Synonyms

Synonyms Comment Organism
elongation factor 1alpha
-
Saccharolobus solfataricus
SsEF-1alpha
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
GTP pH 7.8, 50°C, GTPase activity in the presence 3.6 M NaCl Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00012
-
GDP pH 7.8, 50°C, GTPase activity in the presence 3.6 M NaCl Saccharolobus solfataricus
0.00047
-
guanosine-5'-[(beta,gamma)-imido]triphosphate pH 7.8, 50°C, GTPase activity in the presence 3.6 M NaCl Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.69
-
GTP pH 7.8, 50°C, GTPase activity in the presence 3.6 M NaCl Saccharolobus solfataricus