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Literature summary for 3.6.5.3 extracted from
- The ribosome modulates the structural dynamics of the conserved GTPase HflX and triggers tight nucleotide binding (2012), Biochimie, 94, 1647-1659.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pre-steady-state kinetic analysis of HflX and Hflx ribosomal complexes interacting with GDP and a nonhydrolyzable analogue of mant-GTP, overview | Saccharolobus solfataricus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharolobus solfataricus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50500 | - |
x * 50500, His6-tagged Hflx, SDS-PAGE | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | HflX interacts with 50S and 70S particles, and also with the 30S subunit, independent of the nucleotide-bound state and in tight binding, minimal model for the functional cycle of HflX, interaction with the 70S ribosome and functional mechanism of HflX, overview | Saccharolobus solfataricus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50500, His6-tagged Hflx, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GTPase HflX | - |
Saccharolobus solfataricus |
| HflX | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharolobus solfataricus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | HflX-GTP exists in a structurally distinct 50S- and 70S-bound form that stabilizes GTP binding up to 70000fold and that may represent the GTPase-activated state. This activation is likely required for efficient GTP-hydrolysis, and may be similar to that observed in elongation factor G | Saccharolobus solfataricus |
| physiological function | the universally conserved GTPase HflX is a putative translation factor whose GTPase activity is stimulated by the 70S ribosome as well as the 50S but not the 30S ribosomal subunit | Saccharolobus solfataricus |
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Release 2023.1 (January 2023)
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