Any feedback?
Literature summary for 3.6.5.3 extracted from
- Engineering and characterization of the ribosomal L10.L12 stalk complex. A structural element responsible for high turnover of the elongation factor G-dependent GTPase (2009), J. Biol. Chem., 284, 85-92.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Thermus thermophilus |
| expression in Escherichia coli BL21 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | used as a hybrid sytem with Escherichia coli ribsomal proteins L10, L11, L12 | Thermus thermophilus |
| additional information | used as a hybrid sytem with Thermus thermophilus ribsomal protein L11 | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli | - |
used in hybrid system with Thermus thermophilus ribosomal protein L11 - EC 3.6.5.3 | - |
| Thermus thermophilus | - |
used in hybrid system with Escherichia coli ribosomal proteins L10, L11, L12 - EC 3.6.5.3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE Sephadex A-50 column | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | 0.5 mM GTP, 37°C, 10 min | Thermus thermophilus | GDP + phosphate | - |
? |  |
| GTP + H2O | 0.5 mM GTP, 37°C, 10 min | Escherichia coli | GDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heptamer | - |
Thermus thermophilus |
| pentamer | - |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ec-L10 | - |
Escherichia coli |
| Ec-L11 | - |
Escherichia coli |
| Ec-L12 | - |
Escherichia coli |
| EF-G | - |
Escherichia coli |
| elongation factor | - |
Escherichia coli |
| L10 | - |
Escherichia coli |
| L11 | - |
Thermus thermophilus |
| L11 | - |
Escherichia coli |
| L12 | - |
Escherichia coli |
| Tt-L11 | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Thermus thermophilus |
| 37 | - |
assay at | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
