Protein Variants | Comment | Organism |
---|---|---|
A26G | converts the guanine nucleotide binding consensus sequences A-X-X-X-X-G-K-[T,S] of the elongation factor EF-2 into the corresponding G-X-X-X-X-G-K-[T,S] motif which is present in all the other GTP-binding proteins. In the mutant, the rate of poly(U)-directed poly(Phe) synthesis and the ribosome-dependent GTPase activity of A26GSsEF-2 are decreased. A26G substitution enhances the catalytic efficiency of the intrinsic SsEF-2 GTPase triggered by ethylene glycol and decreases the affinity for GDP | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
GTP | mutant A26G, pH 7.5, 60°C, presence of 40% ethylene glycol | Saccharolobus solfataricus | |
0.001 | - |
GTP | wild-type, pH 7.5, 60°C, presence of 40% ethylene glycol | Saccharolobus solfataricus | |
0.0055 | - |
GTP | mutant A26G, pH 7.5, 60°C, presence of 10% ethylene glycol | Saccharolobus solfataricus | |
0.0096 | - |
GTP | wild-type, pH 7.5, 60°C, presence of 10% ethylene glycol | Saccharolobus solfataricus | |
0.0194 | - |
GTP | mutant A26G, pH 7.5, 60°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Saccharolobus solfataricus | GDP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
elongation factor 2 | - |
Saccharolobus solfataricus |
SsEF-2 | - |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
GTP | wild-type, pH 7.5, 60°C, presence of 10% ethylene glycol | Saccharolobus solfataricus | |
0.032 | - |
GTP | wild-type, pH 7.5, 60°C, presence of 40% ethylene glycol | Saccharolobus solfataricus | |
0.12 | - |
GTP | mutant A26G, pH 7.5, 60°C, presence of 10% ethylene glycol | Saccharolobus solfataricus | |
0.133 | - |
GTP | mutant A26G, pH 7.5, 60°C | Saccharolobus solfataricus | |
0.165 | - |
GTP | mutant A26G, pH 7.5, 60°C, presence of 40% ethylene glycol | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
GDP | wild-type, pH 7.5, 60°C, presence of 40% ethylene glycol | Saccharolobus solfataricus | |
0.0005 | - |
GDP | mutant A26G, pH 7.5, 60°C, presence of 40% ethylene glycol | Saccharolobus solfataricus | |
0.0074 | - |
GDP | mutant A26G, pH 7.5, 60°C | Saccharolobus solfataricus |