Literature summary for 3.6.5.3 extracted from

Laursen, B.S.; Siwanowicz, I.; Larigauderie, G.; Hedegaard, J.; Ito, K.; Nakamura, Y.; Kenney, J.M.; Mortensen, K.K.; Sperling-Petersen, H.U.
Characterization of mutations in the GTP-binding domain of IF2 resulting in cold-sensitive growth of Escherichia coli (2003), J. Mol. Biol., 326, 543-551.

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Protein Variants

Protein Variants	Comment	Organism
A421(insGly)G422	mutation causes cold-sensitivity in the organism. No GTPase activity below 10°C and reduced activity at all temperatures up to 45°C, as compared to wild-type enzyme	Escherichia coli
D409E	mutation causes cold-sensitivity in the organism. No GTPase activity below 10°C and reduced activity at all temperatures up to 45°C, as compared to wild-type enzyme	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Escherichia coli	importance of GTP hydrolysis in translation initiation for optimal cell growth	GDP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Escherichia coli	GDP + phosphate	-	?
GTP + H2O	importance of GTP hydrolysis in translation initiation for optimal cell growth	Escherichia coli	GDP + phosphate	-	?

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Release 2023.1 (January 2023)