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Literature summary for 3.6.5.3 extracted from

  • Harmark, K.; Anborgh, P.H.; Merola, M.; Clark, B.F.C.; Parmeggiani, A.
    Substitution of aspartic acid-80, a residue involved in coordination of Magnesium, weakens the GTP binding and strongly enhances the GTPase of the G domain of elongations factor Tu (1992), Biochemistry, 31, 7367-7372.
    View publication on PubMed
Search for more literature for 3.6.5.3

Activating Compound

Activating Compound Comment Organism Structure
kirromycin
-
 Escherichia coli
NH4+
-
 Escherichia coli
ribosomal subunits
-
 Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
D80N mutant with decreased affinity for GTP and increased GTPase activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 GTP wild type Escherichia coli
0.016
-
 GTP D80N Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Escherichia coli
Li+
-
 Escherichia coli
Mg2+
-
 Escherichia coli
Mn2+
-
 Escherichia coli
Na+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + H2O Escherichia coli
-
 ?
-
 ?
GDP + H2O Escherichia coli N4830
-
 ?
-
 ?
GTP + H2O Escherichia coli
-
 GDP + phosphate
-
 ?
GTP + H2O Escherichia coli N4830
-
 GDP + phosphate
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 elongation factor EF-Tu (wt) and EF-Tu (D80N)
-
Escherichia coli N4830
-
 elongation factor EF-Tu (wt) and EF-Tu (D80N)
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + H2O
-
 Escherichia coli ?
-
 ?
GDP + H2O
-
 Escherichia coli N4830 ?
-
 ?
GTP + H2O
-
 Escherichia coli GDP + phosphate
-
 ?
GTP + H2O
-
 Escherichia coli N4830 GDP + phosphate
-
 ?